ScienCell's GeneQuery™ Human Adipogenesis qPCR Array Kit (GQH-ADP) facilitates gene expression profiling of 88 key genes involved in adipocyte differentiation. Adipocytes are also known as fat cells and are the primary component of adipose tissue, the body's main form of energy storage. There are two types of adipose tissue in mammals comprised of distinct cell populations: white adipocytes that contain a single lipid droplet and brown adipocytes that contain numerous smaller lipid droplets. Examples of how included genes may be classified according to function are shown below:
- Pro-adipogenesis: - LPL, SFRP1, AGT, FABP4, EPAS1
- Anti-adipogenesis: - TRIB3, DDIT3, LRP5, SHH, RORA
- Pro-white adipocytes: - CEBPA. KLF15, SREBF1, EGR2, FGF10
- Pro-brown adipocytes: - IRS2, FOXC2, DIO2, CREB1, UCP1
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg^2+ and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.
this kit (GQH-ADP) facilitates gene expression profiling of 88 key genes involved in adipocyte differentiation.
