Lifeline® Cell Technology distributed in the UK by Caltag Medsystems

Lifeline® Cell Technology – Specialise in Primary Cells and High-Performance Media for the Best Possible Research Outcomes

The founders of Lifeline® Cell Technology have been working in the field of cell culture for over 25 years and continue to be an industry leader by providing unequalled value through meticulous quality testing, constant innovation, and a passion to provide the best customer and technical care.

Lifeline® Cell Technology can provide:

Primary Human Cells
Cell Culture Media
Cell Culture Supplements and Reagents

Primary Human Cells

Lifeline® Cell Technology provides primary human cells that are directly cultured from their source organ tissue, with cells available from the following tissue types: adipose, bladder, blood, bone marrow, breast, cardiac, cardiovascular, eye, kidney, lung-airway, mouth, neural stem cells, male reproductive, female reproductive, skeletal muscle, skin, and umbilical cord.

All of Lifeline® normal and diseased human cells:

Need not be exposed to antimicrobials or phenol red when cultured in Lifeline® culture media. Antimicrobials and phenol red are not needed, and not recommended, to achieve optimal cell performance. These supplements can cause cell stress and “masking effects” that may negatively impact experimental results.
Are negative for mycoplasma, and negative for bacterial and fungal growth.
Are negative for HIV, HBV, and HCB by PCR.

Lifeline® primary human cells include: endothelial, epithelial (including keratinocytes), fibroblasts, hematopoietic, melanocytes, mesenchymal stem cells, neural stem cells, skeletal muscle cells, and smooth muscle cells.

Donor Demographic Information for Human Cells

Need to purchase cells with specific Donor Demographics? Just ask us about Lifeline® Donor Demographic information.

No matter what type of cells you need for your research, you'll find valuable donor information available. You can confidently select what lot best fits your research needs.

You can have donor-specific vials reserved for you for up to 30 days at no charge.
Custom cell isolations: You can have cell lots created specifically for you based on the research needs.
You'll feel confident about publishing your research, because all available lots have donor consent

Lifeline® Donor Demographics include:

Age of donor
Gender of donor
Ethnicity of donor
Smoker vs non-smoker
Cause of Death (if applicable)
Relevant Medical History
Location of Organ
Population Doubling Time for 1^st time out of Cryopreservation
Average Population Doubling Time

Having donor-specific information may help you alleviate additional testing. Donor age and cell passage can affect differentiation in some cases.

To request a list of Donor-Specific Cells, please contact us.

View Products

Lifeline® Cell Culture Media Optimised for Primary Cells for Better Results

Primary Cells cultured in Lifeline^® medium out-perform cells grown in any similar medium.

You'll get consistently reproducible results with Lifeline^® media! Each Lifeline® medium is balanced and supplemented expressly for a particular Primary Cell type, to maximize that individual cell type's life expectancy.

Your cells will plate better using Lifeline^®optimized media (as compared to our nearest competitors' media or traditional high-serum media)
Your cells will grow more quickly in Lifeline^® media than in other commercially-available media
Cells will maintain better morphology when cultured in Lifeline^® media
Lifeline^® media will support the growth of Primary Cells over a period of at least 15 population doublings.
Lifeline^® media contain no antimicrobials and no phenol red (except RPMI) - components which can cause cell stress and “masking effects” that may negatively impact experimental results.
ISO-style rigorous quality control and testing ensures sterility and guaranteed performance.

If you're not achieving optimal results, it may be because you're not using the medium optimised for the specific type of Primary Cells you're working with.

View Products

Cell Culture Supplements and Reagents

Lifeline® Cell Technology can provide:

Antimicrobials
Attachment Factors
Freezing Reagents
Growth Factors and LifeFactors®
Stain Kits
Stem Cell Differentiation Kits
Subculture Reagents
Supplements

View Products

Category	Title
Cell Culture Documents	VascuLife® EnGS-US Endothelial Cell Culture Medium Specification Sheet	View
VascuLife EnGS-US (containing Endothelial Cell Growth Supplement) is a low serum medium optimized for the culture of Human Endothelial Cells including Human Umbilical Vein Endothelial Cells (HUVEC), Aortic Endothelial Cells (AoEC) and other large-vessel endothelial cells. The EnGS in this kit is prepared from bovine brains harvested in the US. VascuLife EnGS-US supports the growth of these cells in a low serum environment without human VEGF.
Cell Culture Documents	VascuLife® EnGS-US Endothelial Cell Culture Medium Instruction Sheet	View
Instruction sheet for VascuLife® EnGS-US Endothelial Cell Culture Medium, product code: LCT-LL-0087.
Primary Cells Documents	Human Skin Cell Systems and Media 2022	View
Lifeline’s Normal Human Skin Cell Systems and optimized Media provide ideal culture models for your critical research of wound healing, skin cancer, burn therapy, dermatological disorders, toxicology, and the screening of cosmetics and skin care products. Additionally, our Xeno-Free Fibroblast cells can be used to establish Xeno-Free human feeder layers for stem cells.
Primary Cells Documents	Human Male Reproductive Cell Systems and Media 2022	View
Lifeline’s Male Reproductive Cells, when grown in Lifeline brand culture media, provide excellent in vitro models for the study of male reproductive biology. Lifeline’s Male Reproductive Cell Systems can be used as normal controls for cancer cell studies. These products are also ideal for the study of androgen receptors and prostate cellular proliferation; the study of reproductive cell morphogenesis; and for cytotoxicity and drug discovery studies.
Primary Cells Documents	Human Lung Cell Systems and Media 2022	View
Lifeline’s Human Lung Cells, when grown in Lifeline brand culture medium, provide ideal culture models for the study of pulmonary diseases such as Asthma, COPD, Cystic Fibrosis, Lung Cancer, as well as the effects of smoking on respiratory health.
Primary Cells Documents	iPS Validated Fibroblast Cell Culture Systems 2022	View
Lifeline’s Normal Human Dermal Fibroblasts have been validated for their ability to become Induced Pluripotent Stem Cells (iPS). Neonatal Fibroblast Cells, LOT # 00866, can be transduced with lentiviral vectors with very high efficiencies.
Primary Cells Documents	Human Mesenchymal Stem Cell (HMSC) Systems and Media 2022	View
Lifeline provides Mesenchymal Stem Cells from different anatomical sites. HMSC-Ad are isolated from adipose tissue. HMSC-BM are isolated from bone marrow tissue. HMSC-WJ are isolated from Wharton’s Jelly of the umbilical cord. HMSC-Pre-Adipocyte are isolated from mature adipocytes.
Primary Cells Documents	Human Fibroblast Cell Systems and Media 2022	View
Lifeline’s Normal Human Fibroblast Cells, when grown in Lifeline brand culture medium, provide ideal cell system to study wound healing, toxicology, cancer, or basic cell biology in various organs including skin, lung, bladder, and the reproductive systems. Our normal Human Fibroblasts can also be used for drug screening, drug development, and genome editing applications. Additionally, our fibroblasts are ideal for establishing serum free human feeder layers for human embryonic stem cells (ESCs), induced pluripotent stem cells (iPSCs), and other cell culture applications requiring feeder layers.
Primary Cells Documents	Human Female Reproductive Cell Systems and Media 2022	View
Lifeline’s Normal Human Female Reproductive Cells, when grown in Lifeline brand culture medium, provide ideal in vitro models for uterine contraction and relaxation studies; uterine, cervical, Fallopian tube, and vaginal cancer studies; general reproductive biology studies; and specific responses to oestrogen and other female reproductive hormones.
Primary Cells Documents	Human Endothelial Cell Systems and Media 2022	View
Lifeline’s Normal Human Endothelial Cells provide an ideal cell system to study angiogenesis, cancer, wound healing, atherosclerosis, inflammation, or vascular biology. Additionally, these cells can also be used for drug development or screening.