UK and Ireland Distributor of Nordic Mubio - Cell Fixation, Permeabilization Kits, high quality antibodies and reagents

FIX&PERM Cell Permeabilization

FIX&PERM Sample Kit

Nordic MuBio

Nordic-MUbio manufacture high quality antibody reagents according to strict ISO 9001 quality guidelines. In December 2013, Nordic-MUbio acquired An Der Grub Bio Research GmbH (ADG), extending their product range with Flow Cytometry products including the 'gold standard' FIX&PERM® Cell Permeabilization Kit.

FIX&PERM® Cell Fixation and Permeabilization Kit

Intracellular staining of protein biomarkers using antibodies in flow cytometry has opened up a diversity of new options for phenotypic and functional characterisation of cells in both research and clinical diagnosis.

Indeed, definitive phenotypic identification of certain cell types can require labelling of both cell surface and intracellular markers. Additionally, the cytoplasmic localisation of typical membrane molecules can prove to be a most reliable lineage marker, such as cytoplasmic CD3 and CD22 in undifferentiated leukaemia.

In such cases, and for many other cell biological and functional investigations, intracellular immunostaining is a necessity. For this purpose Nordic-MUbio offers a class leading cell fixation and permeabilization kit - FIX&PERM® - which offers a number of advantages over other methods for intracellular staining of cells in flow cytometry.

FIX&PERM®

Mildly fixes cells, preserving their flow cytometric scatter characteristics
Allows simultaneous characterisation of both intracellular and cell surface markers
Rapid technique - whole procedure can be carried out in less than one hour, ready for immediate analysis or storage for 24 hours
Stringent QC procedures - the quality of each lot is determined using well-defined blood samples and subsequent comparison of scatter characteristics of obtained leukocyte populations, ensuring consistent and reliable results lot after lot
A range of intracellular antibodies with optimised protocols for use with FIX&PERM®

FIX&PERM® is a simple procedure making use of two reagents. Reagent A gently fixes cells, while Reagent B permeabilizes them. The specific formulations reduce background and allow simultaneous addition of permeabilization medium and fluorochrome labelled antibodies, allowing staining of intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins, etc.

FIX&PERM® Reagents

FIX&PERM Sample Kit (CE), (2 x 5ml), Product Code: GAS-002M

FIX&PERM Kit (CE), (2 x 20ml), Product Code: GAS-002

FIX&PERM Kit 1000 (CE), (2 x 100ml), Product Code: GAS-002-1

FIX&PERM Solution A (Fix) (CE), (1 x 100ml), Product Code: GAS-002A-1

FIX&PERM Solution B (Perm) (CE), (1 x 100ml), product Code: GAS-002B-1

FIX&PERM Test Kit, (2 x 1.5 ml), Product Code: GAS-002FOC

Products optimised for use with FIX&PERM®

The majority of available monoclonal antibody conjugates can be used with FIX&PERM®. However, in some cases the fixation step may have to be optimised.

Nordic-MUbio offers a number of mouse anti-human conjugated (PE/FITC) monoclonal antibodies optimised for use with FIX&PERM®.

Antigen	Clone	Isotype	Format	Quantity	Cat. No.
CD3	UCHT1	IgG1	Purified	0.2 mg	GM-4011
			FITC-labeled	100 tests	GM-4012
			PE-labeled	100 tests	GM-4013
CD22	RFB4	IgG1	Purified	0.2 mg	GM-4051
			FITC-labeled	100 tests	GM-4052
			PE-labeled	100 tests	GM-4053
Lactoferrin	4C5	IgG1	Purified	0.2 mg	GM-4111
			PE-labeled	100 tests	GM-4113
Myeloperoxidase	8E6	IgG1	Purified	0.2 mg	GM-4191
(MPO-C2)			FITC-labeled	100 tests	GM-4192
			PE-labeled	100 tests	GM-4193
Lysozyme	LZ-2	IgG1	Purified	0.2 mg	GM-4131
			FITC-labeled	100 tests	GM-4132
CD68	Ki-M7	IgG1	FITC-labeled	100 tests	GM-4152
IL-1 beta	FIB3	IgG1	FITC-labeled	100 tests	GM-4162
IFN-gamma	GZ4	IgG1	FITC-labeled	100 tests	GM-4172

Furthermore, a number of Combi-panels are offered for dual immunostaining protocols.

Antigen	Clone	Isotype	Format	Quantity	Cat. No.
Negative control	Vl-AP	IgG1	FITC-labeled	50 tests	GIC-201
	Vl-AP	IgG1	PE-labeled
Lysozyme	LZ-2	IgG1	FITC-labeled	50 tests	GIC-206
Lactoferrin	4C5	IgG1	PE-labeled
Myeloperoxidase	8E6	IgG1	FITC-labeled	50 tests	GIC-212
Lactoferrin	4C5	IgG1	PE-labeled
Myeloperoxidase	8E6	IgG1	FITC-labeled	50 tests	GIC-213
CD3	UCHT1	IgG1	PE-labeled
Myeloperoxidase	8E6	IgG1	FITC-labeled	50 tests	GIC-214
CD22	RFB4	IgG1	PE-labeled

Documents

Category	Title
Antibodies Documents	Rabbit anti-rat CD90/Thy-1 (MUB2058)	View
Nordic-MUbio is pleased to announce the release of one of the original antibodies (MUB2058) developed for the identification and characterisation of Thy-1 (CD90) in rats, used in some of the highly cited work of Barclay et al.
Kits Documents	FIX&PERM® Cell Fixation and Permeabilisation Kit	View
FIX&PERM® is a simple procedure making use of two reagents. Reagent A gently fixes cells, while Reagent B permeabilises them.
Antibodies Documents	Scicons double-stranded RNA detection	View
Demand for antibodies against dsRNA continues to grow with the success of mRNA vaccines in the fight against COVID-19. Current mRNA technology, where mRNA is transcribed from cloned DNA by the T7 RNA polymerase, results in the creation of dsRNA impurities. To monitor the removal of this unwanted dsRNA by-product before use, Scicons antibodies are the gold standard.
Antibodies Documents	Veterinary Immunoglobulins	View
Nordic-MUbio is dedicated to helping veterinary scientists advance their understanding of animal immunology, helping to safeguard everything from domestic pets to livestock in health and disease.
Antibodies Documents	Exalpha	View
Antibodies and Reagents that Answer Real Needs. FIX&PERM, BrdU, SPARC, Chicken IgY, Custom Chicken IgY Services.
Antibodies Documents	Eggcellent Antibodies- Chicken IgY	View
Utilising Gallus' eggspertise, Nordic-MUbio are now able to offer a greatly expanded range of antibodies generated in chicken. Polyclonal Antibodies generated in chickens, offer many advantages over Polyclonal Antibodies traditionally produced in mammals such as rabbits, goats and sheep. Chickens produce immunoglobulin not only in their serum, but also in the egg yolk. The concentration of immunoglobulin measured in egg yolks has been reported to be higher than that found in mammalian and chicken serum. Chickens are more apt to make high-avidity antibodies compared to mammalian antigens.
Antibodies Documents	Green Fluorescent Protein (GFP) Antibodies	View
Our new range of anti-GFP antibodies bring a number of advantages over antibodies produced against recombinant, or variant, forms of GFP.
Immunology Documents	Human Immunoglobulins	View
Nordic-MUbio oers the highest quality range of products for the study and analysis of human immunoglobulins