NSJ Bioreagents

PKC beta 1 Antibody / PRKCB1

Product Code:
 
NSJ-RQ4656
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the PKC beta antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.
1 / 7
Western blot testing of human 1) K562, 2) THP-1, 3) HEK293, 4) Jurkat, 5) Raji, 6) HL-60 and 7) A549 lysate with PKC beta antibody at 0.5ug/ml. Predicted molecular weight ~76 kDa.
2 / 7
Western blot testing of rat 1) thymus, 2) spleen, 3) brain, 4) C6 cells, and mouse 5) thymus, 6) spleen, 7) brain, 8) Neuro-2a cells and 9) NIH 3T3 cell lysate with PKC beta antibody at 0.5ug/ml. Predicted molecular weight ~76 kDa.
3 / 7
IHC staining of FFPE human intestinal cancer with PKC beta antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
4 / 7
IHC staining of FFPE human prostate cancer with PKC beta antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
5 / 7
IHC staining of FFPE human placenta with PKC beta antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
6 / 7
IHC staining of FFPE mouse small intestine with PKC beta antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
7 / 7
IHC staining of FFPE rat lung with PKC beta antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
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Western blot testing of human 1) K562, 2) THP-1, 3) HEK293, 4) Jurkat, 5) Raji, 6) HL-60 and 7) A549 lysate with PKC beta antibody at 0.5ug/ml. Predicted molecular weight ~76 kDa.
Western blot testing of rat 1) thymus, 2) spleen, 3) brain, 4) C6 cells, and mouse 5) thymus, 6) spleen, 7) brain, 8) Neuro-2a cells and 9) NIH 3T3 cell lysate with PKC beta antibody at 0.5ug/ml. Predicted molecular weight ~76 kDa.
IHC staining of FFPE human intestinal cancer with PKC beta antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC staining of FFPE human prostate cancer with PKC beta antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC staining of FFPE human placenta with PKC beta antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC staining of FFPE mouse small intestine with PKC beta antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC staining of FFPE rat lung with PKC beta antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

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CodeSizePrice
NSJ-RQ4656-100ug100ug£535.00
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Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the PKC beta antibody should be determined by the researcher.
Description:
Protein kinase C beta type is an enzyme that in humans is encoded by the PRKCB gene. It is a member of the protein kinase C (PKC) gene family. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. This protein kinase has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. It has been found that PRKCB activated by oxidative conditions in the cell, induces phosphorylation of p66(SHC) and triggers mitochondrial accumulation of the protein after it is recognized by the prolyl isomerase PIN1.
Format :
Purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids NFDKEFTRQPVELTPTDKLFIMNLDQNE were used as the immunogen for the PKC beta antibody.
Limitation:
This PKC beta antibody is available for research use only.
Localization:
Nuclear, cytoplasmic, membrane
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P05771