ProSci

LAG3 Antibody [1G4]

Product Code:
 
PSI-RF16089
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Mouse
Antibody Isotype:
 
IgG1,k
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
1G4
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunocytochemistry (ICC)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Shipping:
 
blue ice
Storage:
 
LAG-3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of LAG-3 in over expressing HEK293 cells using LAG-3 antibody at (A) 0.25 (B) 0.5 and (C) 1 μg/ml.
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Immunocytochemistry of LAG-3 in over expressing HEK293 cells using LAG-3 antibody and control mouse IgG antibody (left corner box) at 1 μg/ml.
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Immunofluorescence of LAG-3 in over expressing HEK293 cells using LAG-3 Antibody at 2 μg/ml.<br><br>Green: LAG3 Antibody [1G4] (RF16089) <br> Blue: DAPI staining
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Immunofluorescence of LAG-3 in over human spleen tissue using LAG-3 Antibody at 10 μg/ml.<br><br>Green: LAG3 Antibody [1G4] (RF16089) <br> Blue: DAPI staining
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Immunohistochemistry of LAG-3 in human lymphoma tissue using LAG-3 Antibody and control mouse IgG (corner box) at 5 μg/ml.
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Flow cytometry analysis of LAG-3 over expressing HEK293 cells using LAG-3 antibody at 1 μg/ml. Blue: untransfected HEK293 cells. Yellow: LAG-3 over expressing HEK293 cells.
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Titration curve analysis of LAG-3 mAbs to detect recombinant LAG-3 in ELISA with RF16082, RF16083, RF16084, RF16086, RF16087, RF16088, and RF16089 antibodies at decreasing concentrations.
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A sandwich ELISA was performed using the anti-LAG3 mAbs RF16082, RF16083, RF16084, RF16086, RF16087 and RF16088 as the capture antibodies for the LAG3 extracellular domain antigen with biotin-labeled Risk-Free anti-LAG3 mAbs as the detection antibodies.
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Western blot analysis of LAG-3 in over expressing HEK293 cells using LAG-3 antibody at (A) 0.25 (B) 0.5 and (C) 1 μg/ml.
Immunocytochemistry of LAG-3 in over expressing HEK293 cells using LAG-3 antibody and control mouse IgG antibody (left corner box) at 1 μg/ml.
Immunofluorescence of LAG-3 in over expressing HEK293 cells using LAG-3 Antibody at 2 μg/ml.<br><br>Green: LAG3 Antibody [1G4] (RF16089) <br> Blue: DAPI staining
Immunofluorescence of LAG-3 in over human spleen tissue using LAG-3 Antibody at 10 μg/ml.<br><br>Green: LAG3 Antibody [1G4] (RF16089) <br> Blue: DAPI staining
Immunohistochemistry of LAG-3 in human lymphoma tissue using LAG-3 Antibody and control mouse IgG (corner box) at 5 μg/ml.
Flow cytometry analysis of LAG-3 over expressing HEK293 cells using LAG-3 antibody at 1 μg/ml. Blue: untransfected HEK293 cells. Yellow: LAG-3 over expressing HEK293 cells.
Titration curve analysis of LAG-3 mAbs to detect recombinant LAG-3 in ELISA with RF16082, RF16083, RF16084, RF16086, RF16087, RF16088, and RF16089 antibodies at decreasing concentrations.
A sandwich ELISA was performed using the anti-LAG3 mAbs RF16082, RF16083, RF16084, RF16086, RF16087 and RF16088 as the capture antibodies for the LAG3 extracellular domain antigen with biotin-labeled Risk-Free anti-LAG3 mAbs as the detection antibodies.

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CodeSizePrice
PSI-RF16089-0.02mg0.02mg£150.00
Quantity:
PSI-RF16089-0.1mg0.1mg£515.00
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Further Information

Additional Names:
LAG3 Antibody: lymphocyte activating 3, LAG-3, CD223
Application Note:
LAG-3 antibody can be used for ELISA starting at 0.25 μg/mL. For Western blot start at 0.25 μg/mL. For Immunocytochemistry start at 1 μg/mL. For Immunofluorescence start at 10 μg/mL. For Immunohistochemistry start at 5 μg/mL. For Flow Cytometry start at 1 μg/ml.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples and Flow Cytometry in human samples. All other applications and species not yet tested.
Background:
The lymphocyte activation gene-3 (LAG3) is a member of the immunoglobulin superfamily and binds MHC class II with high affinity (1), negatively regulating T-cell function and homeostasis (2). It is expressed in B, T, and NK cells, monocytes, and dendritic cells (3), and acts to regulate T cell expansion (4). LAG3 is also an important immune checkpoint protein, with anti-LAG3 antibodies activating T effector cells and affecting regulatory T cell functions. Furthermore LAG3 appears to act in a synergistic fashion with PD-1/PD-L1, suggesting that a dual antibody approach may prove useful in cancer immunotherapy.
Background References:
  • Huard B, Tournier M, Hercend T, et al. Lymphocyte-activation gene 3/major histocompatibility complex class II interaction modulates the antigenic response of CD4+ T lymphocytes. Eur J Immunol 1994; 24:3216?21.
  • Triebel F. LAG-3: a regulator of T-cell and DC responses and its use in therapeutic vaccination. Trends Immunol 2003; 24:619?22.
  • Workman CJ, Wang Y, El Kasmi KC, et al. LAG-3 regulates plasmacytoid dendritic cell homeostasis. J Immunol. 2009; 182:1885?91.
  • Workman CJ, Vignali DA. The CD4-related molecule, LAG-3 (CD223), regulates the expansion of activated T cells. Eur J Immunol 2003; 33:970?9.
Buffer:
LAG-3 Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
LAG-3 antibody was raised against the extracellular domain of human LAG-3
NCBI Gene ID #:
3902
NCBI Official Name:
lymphocyte activating 3
NCBI Official Symbol:
LAG3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 55 kDa

Observed: 60, 70, 80 kDa
Protein Accession #:
NP_002277
Protein GI Number:
167614500
Purification:
LAG-3 Antibody is supplied as protein A purified IgG1.
Research Area:
Immunology
Swissprot #:
P18627
User NOte:
Optimal dilutions for each application to be determined by the researcher.