ProSci

CD63 Antibody [MX-49.129.5]

Product Code:
 
PSI-33-100
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Mouse
Antibody Isotype:
 
IgG1, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
MX-49.129.5
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
 
blue ice
Storage:
 
Aliquot and Store at -20C. Avoid freez-thaw cycles.
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IHC testing of human melanoma stained with CD63 antibody (MX49.129.5).
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FACS testing of mouse NIH3T3: Black=cells alone; Green=isotype control; Red=CD63 antibody PE conjugate
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Western blot testing of human spleen lysate with CD63 antibody at 2 ug/ml.
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IHC testing of FFPE prostate carcinoma with CD63 antibody.
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Immunofluorescence testing of HeLa cells with Alexa Fluor 488 conjugated CD63 antibody (green). F-actin filaments are labeled with Dylight 554 phalloidin (red); nuclei stained with DAPI (blue).

IHC testing of human melanoma stained with CD63 antibody (MX49.129.5).
FACS testing of mouse NIH3T3: Black=cells alone; Green=isotype control; Red=CD63 antibody PE conjugate
Western blot testing of human spleen lysate with CD63 antibody at 2 ug/ml.
IHC testing of FFPE prostate carcinoma with CD63 antibody.
Immunofluorescence testing of HeLa cells with Alexa Fluor 488 conjugated CD63 antibody (green). F-actin filaments are labeled with Dylight 554 phalloidin (red); nuclei stained with DAPI (blue).

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PSI-33-100-100ug100ug£765.00
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Further Information

Additional Names:
CD63 antigen, Granulophysin, Lysosomal-associated membrane protein 3, LAMP-3, Melanoma-associated antigen ME491, OMA81H, Ocular melanoma-associated antigen, Tetraspanin-30, Tspan-30, CD63, CD63, MLA1, TSPAN30
Application Note:
Flow Cytometry: 0.5-1 ug/million cells

IF: 0.5-1 ug/ml

WB: 1-2 ug/ml

IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)

Prediluted format : incubate for 30 min at RT (2)

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Background:
The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multi-molecular complexes with specific integrins. The tetraspanin CD63 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. CD63 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. CD63 deficiency is associated with Hermansky-Pudlak syndrome and is strongly expressed during the early stages of melanoma progression.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Full length human CD63 was used as the immunogen for this antibody.
NCBI Gene ID #:
967
NCBI Official Name:
CD63 molecule
NCBI Official Symbol:
CD63
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Cancer,Immunology
Swissprot #:
P08962
User NOte:
Optimal dilutions for each application to be determined by the researcher