ProSci

Recombinant CD44v4 Antibody [CD44v4/1700R]

Product Code:
 
PSI-34-065
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG, kappa
Antibody Clonality:
 
Recombinant Antibody
Antibody Clone:
 
CD44v4/1700R
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Shipping:
 
blue ice
Storage:
 
Aliquot and Store at -20C. Avoid freez-thaw cycles.
1 / 1
IHC testing of FFPE human tonsil with recombinant CD44v4 antibody (clone CD44v4/1700). Required HIER: steam sections in pH6 citrate buffer for 20 min.

IHC testing of FFPE human tonsil with recombinant CD44v4 antibody (clone CD44v4/1700). Required HIER: steam sections in pH6 citrate buffer for 20 min.

No additional charges, what you see is what you pay! *

CodeSizePrice
PSI-34-065-100ug100ug£765.00
Quantity:
Prices exclude any Taxes / VAT
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: United States.
Typical lead time: 14-21 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • Show All

Further Information

Additional Names:
CD44, CD44 molecule (Indian blood group), CDW44, CSPG8, ECMR-III, HCELL, HUTCH-I, IN, LHR, MC56, MDU2, MDU3, MIC4, Pgp1, CD44 antigen, GP90 lymphocyte homing/adhesion receptor, Hermes antigen, cell surface glycoprotein CD44, chondroitin sulfate proteoglyc
Application Note:
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml

Immunofluorescence: 0.5-1 ug/ml

Immunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT

Prediluted IHC only format: incubate for 30 min at RT (1)

Titering of the recombinant CD44v4 antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Background:
This antibody recognizes an epitope encoded by exon v4 on the variant portion of human CD44. The CD44 molecule belongs to a family of cellular adhesion molecules found on a wide range of normal and malignant cells in epithelial, mesothelial and hemopoiesis tissues. It is a single gene with 20 exons, of which 10 are normally expressed to encode the basic CD44 (H-CAM) molecule. The additional 10 exons (v1 to v10) are only expressed by alternative splicing of the nuclear RNA. The expression of specific cell adhesion molecule CD44 splice variants has been reported to be associated with metastasis in certain human malignancies.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
An amino acid sequence from the variant 4 domain of CD44 was used as the immunogen for the recombinant CD44v4 antibody.
NCBI Gene ID #:
960
NCBI Official Name:
CD44 molecule (Indian blood group)
NCBI Official Symbol:
CD44
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein A affinity chromatography
Research Area:
Signal Transduction,Signal Transduction
Swissprot #:
P16070
User NOte:
Optimal dilutions for each application to be determined by the researcher