Further Information
Follitropin subunit beta, Follicle-stimulating hormone beta subunit, FSH-B, FSH-beta, Follitropin beta chain, FSHB
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
Immunofluorescence: 1-2 ug/ml
Immunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min RT (1)
Prediluted format : incubate for 30 min at RT (2)
Optimal dilution of the FSH-beta antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
This mAb reacts with a protein of 22kDa, identified as beta sub-unit of FSH. It does not cross react with the alpha sub-unit. Follicle stimulating hormone (FSH) is a hormone synthesized and secreted by gonadotrophs in the anterior pituitary gland. In the ovary, FSH stimulates the growth of immature Graafian follicles to maturation. As the follicle grows, it releases inhibin, which deactivates the FSH production. In men, FSH enhances the production of androgen-binding protein by the Sertoli cells of the testis and is critical for spermatogenesis. FSH and LH act synergistically in reproduction. FSH is a useful marker in the classification of pituitary tumors and the study of pituitary disease.
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
0.2 mg/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Full length purified hFSH was used as the immunogen for the FSH-beta antibody.
2488
follicle stimulating hormone, beta polypeptide
FSHB
Homo sapiens
Liquid
Protein G affinity chromatography
Cancer,Cell Cycle,Obesity,Signal Transduction
P01225
Optimal dilutions for each application to be determined by the researcher
