ProSci

TLR2 Antibody [TLR2/221]

Product Code:
 
PSI-33-471
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Mouse
Antibody Isotype:
 
IgG2a, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
TLR2/221
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Monkey
Applications:
  • Functional Study
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Shipping:
 
blue ice
Storage:
 
Aliquot and Store at -20C. Avoid freez-thaw cycles.
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SDS-PAGE Analysis of Purified, BSA-Free TLR2 Antibody (clone TLR2/221). Confirmation of Integrity and Purity of the Antibody.
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SDS-PAGE Analysis of Purified, BSA-Free TLR2 Antibody (clone TLR2/221). Confirmation of Integrity and Purity of the Antibody.

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PSI-33-471-100ug100ug£765.00
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Further Information

Additional Names:
TLR2 Antibody: TIL4, CD282, TIL4, Toll-like receptor 2
Application Note:
Functional Activity (Order SAF formulation)

Flow Cytometry: 0.5-1 ug/million cells in 0.1ml

Immunofluorescence: 1-2 ug/ml

Immunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)

Optimal dilution of the TLR2 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.

Background:
This mAb reacts with human Toll-like receptor 2 (TLR2). It is a member of the Toll-like receptor (TLR) family, which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is expressed most abundantly in peripheral blood leukocytes, and mediates host response to Gram-positive bacteria and yeast via stimulation of NF-kappaB.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant human protein was used as the immunogen for the TLR2 antibody.
NCBI Gene ID #:
7097
NCBI Official Name:
toll-like receptor 2
NCBI Official Symbol:
TLR2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Innate Immunity
Swissprot #:
O60603
User NOte:
Optimal dilutions for each application to be determined by the researcher