ProSci

CELA3B Antibody [CELA3B/1757]

Product Code:
 
PSI-33-794
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Mouse
Antibody Isotype:
 
IgG1, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
CELA3B/1757
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Shipping:
 
blue ice
Storage:
 
Aliquot and Store at -20C. Avoid freez-thaw cycles.
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IHC testing of human pancreas with CELA3B antibody (clone CELA3B/1757). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.
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IHC testing of human pancreas with CELA3B antibody (clone CELA3B/1757). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.

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PSI-33-794-100ug100ug£765.00
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Further Information

Additional Names:
Chymotrypsin-like elastase family member 3B, Elastase IIIB, Elastase-3B, Protease E, CELA3B, ELA3B
Application Note:
Western blot: 0.5-1 ug/ml

Flow Cytometry: 0.5-1 ug/million cells in 0.1ml

IF: 1-2 ug/ml

ELISA: 2-4 ug/ml; order BSA free formulation for coating

IHC (FFPE): 1-2 ug/ml for 30 min at RT

Optimal dilution of the CELA3B antibody should be determined by the researcher.
Background:
This mAb recognizes a protein of ~30kDa, identified as CELA3B (Chymotrypsin like elastase family member 3B), or Elastase 3B. Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six genes which encode the structurally similar proteins: Elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, 3B has little elastolytic activity. Like most of the human elastases, 3B is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein; it has a digestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. It may also function in the intestinal transport and metabolism of cholesterol. Elastases 3A/B have been referred to as Protease E and as Elastase 1, and excretion of this protein in fecal material is frequently used as a measure of pancreatic function in clinical assays.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A recombinant partial protein (aa 82-238) was used as the immunogen for the CELA3B antibody.
NCBI Gene ID #:
23436
NCBI Official Name:
Chymotrypsin-like elastase family member 3B
NCBI Official Symbol:
CELA3B
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Cell Cycle,Signal Transduction
Swissprot #:
P08861
User NOte:
Optimal dilutions for each application to be determined by the researcher