ProSci

CD56 Antibody [SPM489]

Product Code:
 
PSI-34-141
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Mouse
Antibody Isotype:
 
IgG1, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
SPM489
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
Shipping:
 
blue ice
Storage:
 
Aliquot and Store at -20C. Avoid freez-thaw cycles.
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Formalin-fixed, paraffin-embedded human colon stained with anti-CD56 antibody (SPM489)
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Formalin-fixed, paraffin-embedded human colon stained with anti-CD56 antibody (SPM489)

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PSI-34-141-100ug100ug£765.00
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Further Information

Additional Names:
neural cell adhesion molecule, NCAM, antigen recognized by monoclonal 5.1H11, NCAM-1, N-CAM-1, MSK39, CD56, neural cell adhesion molecule 1, NCAM1
Application Note:
Immunofluorescence: 1-2 ug/ml

Immunohistochemistry (FFPE): 1-2 ug/ml for 30 minutes at RT (1)

Prediluted format: incubate for 30 min at RT (2)

The optimal dilution of the anti-CD56 antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Background:
Three isoforms of neural cell adhesion molecule (NCAM) are produced by differential splicing of the RNA transcript from a single gene. The 135kDa isoform is the basic molecule, which is glycosylated or sialylated to produce the mature species. Anti-CD56 recognizes two proteins of the neural cell adhesion molecule, the basic molecule expressed on most neuroectodermally derived tissues and neoplasms (e.g. retinoblastoma, medulloblastomas, astrocytomas, neuroblastomas, and small cell carcinomas). It is also expressed on some mesodermally derived tumors (rhabdomyosarcoma). Anti-CD56 plays an important role in the diagnosis of nodal and nasal NK/T-cell lymphomas.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A membrane preparation of small cell lung carcinoma was used as the immunogen for this anti-CD56 antibody. This mAb reacts with an extracellular domain (close to transmembrane) of CD56/NCAM.
NCBI Gene ID #:
4684
NCBI Official Name:
neural cell adhesion molecule 1
NCBI Official Symbol:
NCAM1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Neuroscience, Stem Cell
Swissprot #:
P13591
User NOte:
Optimal dilutions for each application to be determined by the researcher