Rabbit anti Human Phosphatidic Acid Phosphatase 2B

Product Code:

EXA-X1651P

Product Group:

Primary Antibodies

Supplier:

Nordic MuBio

Host Type:

Rabbit

Antibody Isotype:

IgG

Antibody Clonality:

Polyclonal

Regulatory Status:

RUO

Target Species:

Human
Mouse
Rat

Applications:

Enzyme Immunoassay (EIA)
Western Blot (WB)

Shipping:

Ship on dry ice freeze upon arrival

Storage:

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

No additional charges, what you see is what you pay! *

Code	Size	Price

EXA-X1651P

100 ug

£316.00

Quantity:

Prices exclude any Taxes / VAT

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Further Information

Applications Description:

Antibody can be used for Western blotting (see vial for dilution) and EIA. Optimal concentration should be evaluated by serial dilutions.

Background:

Vascular endothelial growth factor and type I collagen inducible protein (VCIP), also known as phosphatidic acid phosphatase 2b (PAP2b), was originally identified in a functional assay of angiogenesis. VCIP/PAP2b exhibits an Arg?Gly?Asp (RGD) cell adhesion sequence. Immunoprecipitation and fluorescence-activated cell sorting analyses demonstrated that VCIP-RGD is exposed to the outside of the cell surface. Retroviral transduction of VCIP can induce cell aggregation and cell? cell interactions, modestly increase p120 catenin expression and promote activation of the Fak, Akt and GSK3? protein kinases. Expression of VCIP promoted adhesion, spreading and tyrosine phosphorylation of Fak, Shc, Cas and paxillin in endothelial cells. PAP2b/VCIP is expressed in close proximity to vascular endothelial growth factor, von Willebrand factor and {alpha}v?3 integrin in tumor vasculatures.

Caution:

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

Concentration:

Lot specific, see vial

Field of Interest:

Signal Transduction

Formulation:

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Functional Analysis:

Western Blotting

Immunogen:

Synthetic peptide derived from the phosphatidic acid phosphatase 2B protein

Positive Control:

Human brain lysate (Cat. No. X1633C)

Product Form:

Unconjugated

Product Stability:

Products are stable for one year from purchase when stored properly

Purification Method:

Antigen Immunoaffiinity Purification

Synonyms:

PAP2B

UniProt:

O14495

Documents

Further Information

References

[1] Kai M., Wada I., Imai S.-I., Sakane F., Kanoh H.; Cloning and characterization of two human isozymes of Mg2+-independent phosphatidic acid phosphatase.; J. Biol. Chem. 272:24572-24578(1997).

[2] Roberts R., Sciorra V.A., Morris A.J.; Human type 2 phosphatidic acid phosphohydrolases. Substrate specificity of the type 2a, 2b, and 2c enzymes and cell surface activity of the 2a isoform.; J. Biol. Chem. 273:22059-22067(1998).

[3] Humtsoe J.O., Feng S., Thakker G.D., Yang J., Hong J., Wary K.K.; Regulation of cell-cell interactions by phosphatidic acid phosphatase 2b/VCIP.; EMBO J. 22:1539-1554(2003).

[4] Leung D.W., Tompkins C.K.; Molecular cloning of and expression of an isoform of human phosphatidic acid phosphatase cDNA.; Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases.

[5] Yu W., Andersson B., Worley K.C., Muzny D.M., Ding Y., Liu W., Ricafrente J.Y., Wentland M.A., Lennon G., Gibbs R.A.; Large-scale concatenation cDNA sequencing.;
Genome Res. 7:353-358(1997).

[6] Strausberg R.L., et al Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.; Proc. Natl. Acad. Sci. U.S.A. 99:16899-16903(2002).

[7] Burnett C., Makridou P., Hewlett L., Howard K.; Lipid phosphate phosphatases dimerise, but this interaction is not required for in vivo activity.; BMC Biochem. 5:2-2(2004).