Nordic MuBio

Mouse anti Phosphotyrosine conjugated with biotin

Product Code:
 
EXA-X1016S
Product Group:
 
Primary Antibodies
Supplier:
 
Nordic MuBio
Host Type:
 
Mouse
Antibody Isotype:
 
IgG2b
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
PY20
Regulatory Status:
 
RUO
Target Species:
 
Species Independent
Applications:
  • Enzyme Immunoassay (EIA)
  • Immunohistochemistry (IHC)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Shipping:
 
Room Temperature
Storage:
 
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
 

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EXA-X1016S50 ug£196.00
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Further Information

Applications Description:
Application: A dilution of 1:5,000 is suggested for western blot or ELISA, 1:500 for immunohistochemistry or immunocytochemistry.
Background:
The role of tyrosine phosphorylation in transduction of the mitogenic signal from transmembrane receptors and in transformation by oncogene tyrosine kinases has been the subject of intense investigation for several years. While the phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine-phosphorylated proteins biochemical pathways in which they function. The biotin conjugate of clone PY20 anti-phosphotyrosine is especially useful for the detection of phosphotyrosyl proteins in western blotting, immunohistochemical and immunocytochemical protocols in situations wherein the use of a secondary antibody would produce unacceptable background signals. In addition, the strength of the biotin-streptavidin interaction and the immense variety of streptavidin-coupled probes available make PY20-biotin a very versatile reagent.
Caution:
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
Concentration:
See vial for concentration
Field of Interest:
Signal Transduction
Formulation:
Provided as sterile filtered solution containing 20 mM sodium phosphate, 150 mM sodium chloride, 50% glycerol at pH 7.5 and 3 mM sodium azide
Functional Analysis:
Western Blotting
Immunogen:
Hybridoma produced from Balb/C mice immunized with phosphotyrosine coupled to carrier protein.
Label:
Biotin
Product Form:
Biotin
Product Stability:
Products are stable for one year from purchase when stored properly
Purification Method:
Prot A/G
Source:
Hybridoma produced from Balb/C mice immunized with phosphotyrosine coupled to carrier protein.
Synonyms:
Mouse anti phosphotyrosine, PY20, Biotin Conjugate
UniProt:
N/A