ProSci

CASP12 Antibody

Product Code:
 
PSI-13-082
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 5
Western blot analysis of extracts of various cell lines, using CASP12 Antibody (13-082) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 5
Immunohistochemistry of paraffin-embedded human prostate using CASP12 antibody (13-082) at dilution of 1:100 (20x lens).
3 / 5
Immunohistochemistry of paraffin-embedded human stomach using CASP12 antibody (13-082) at dilution of 1:100 (40x lens).
4 / 5
Immunofluorescence analysis of NIH-3T3 cells using CASP12 Polyclonal Antibody (13-082) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
5 / 5
Immunofluorescence analysis of U-2 OS cells using CASP12 Polyclonal Antibody (13-082) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using CASP12 Antibody (13-082) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human prostate using CASP12 antibody (13-082) at dilution of 1:100 (20x lens).
Immunohistochemistry of paraffin-embedded human stomach using CASP12 antibody (13-082) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of NIH-3T3 cells using CASP12 Polyclonal Antibody (13-082) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using CASP12 Polyclonal Antibody (13-082) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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PSI-13-082-100uL100 uL£565.00
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Further Information

Additional Names:
CASP12, CASP-12, CASP12P1, caspase 12 (gene/pseudogene), caspase 12 pseudogene 1, inactive caspase-12, OTTHUMP00000207026, OTTHUMP00000207028, OTTHUMP00000207029, OTTHUMP00000207031, OTTHUMP00000207032, OTTHUMP00000219058
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:100
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CASP12 (NP_001177945.2).
NCBI Gene ID #:
100506742
NCBI Official Name:
caspase 12 (gene/pseudogene)
NCBI Official Symbol:
CASP12
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 42kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cancer, Cell Cycle, Neuroscience
Swissprot #:
Q6UXS9
User NOte:
Optimal dilutions for each application to be determined by the researcher.