ProSci

KU70 Antibody

Product Code:
 
PSI-13-312
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of various cell lines, using KU70 antibody (13-312) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 60s.
2 / 6
Immunohistochemistry of paraffin-embedded rat brain using KU70 Antibody (13-312) at dilution of 1:100 (40x lens).
3 / 6
Immunohistochemistry of paraffin-embedded human breast using KU70 Antibody (13-312) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human leiomyoma of uterus using KU70 Antibody (13-312) at dilution of 1:100 (40x lens).
5 / 6
Immunohistochemistry of paraffin-embedded mouse lung using KU70 Antibody (13-312) at dilution of 1:100 (40x lens).
6 / 6
Immunofluorescence analysis of HeLa cells using KU70 antibody (13-312) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using KU70 antibody (13-312) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 60s.
Immunohistochemistry of paraffin-embedded rat brain using KU70 Antibody (13-312) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast using KU70 Antibody (13-312) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human leiomyoma of uterus using KU70 Antibody (13-312) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using KU70 Antibody (13-312) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using KU70 antibody (13-312) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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PSI-13-312-100uL100 uL£565.00
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Further Information

Additional Names:
G22P1, ML8, KU70, TLAA, CTC75, CTCBF, G22P1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
The p70/p80 autoantigen is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 500-600 of human KU70 (NP_001460.1).
NCBI Gene ID #:
2547
NCBI Official Name:
X-ray repair complementing defective repair in Chinese hamster cells 6
NCBI Official Symbol:
XRCC6
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 70kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle
Swissprot #:
P12956
User NOte:
Optimal dilutions for each application to be determined by the researcher.