ProSci

KLF6 Antibody

Product Code:
 
PSI-13-372
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 2
Western blot analysis of extracts of PC-3 cells, using KLF6 antibody (13-372) at 1:500 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
2 / 2
Immunofluorescence analysis of HeLa cells using KLF6 antibody (13-372) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of PC-3 cells, using KLF6 antibody (13-372) at 1:500 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
Immunofluorescence analysis of HeLa cells using KLF6 antibody (13-372) at dilution of 1:100. Blue: DAPI for nuclear staining.

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PSI-13-372-100uL100 uL£565.00
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Further Information

Additional Names:
KLF6, BCD1, COPEB, CPBP, DKFZp686N0199, GBF, PAC1, ST12, ZF9, CBA1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:100
Background:
This gene encodes a member of the Kruppel-like family of transcription factors. The zinc finger protein is a transcriptional activator, and functions as a tumor suppressor. Multiple transcript variants encoding different isoforms have been found for this gene, some of which are implicated in carcinogenesis.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 40-200 of human KLF6 (NP_001291.3).
NCBI Gene ID #:
1316
NCBI Official Name:
Kruppel-like factor 6
NCBI Official Symbol:
KLF6
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 45kDa
Purification:
Affinity purification
Research Area:
Stem Cell, Transcription
Swissprot #:
Q99612
User NOte:
Optimal dilutions for each application to be determined by the researcher.