ProSci

GPER1 Antibody

Product Code:
 
PSI-13-509
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 4
Western blot analysis of extracts of various cell lines, using GPER1 antibody (13-509) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 4
Immunofluorescence analysis of C6 cells using GPER1 antibody (13-509) at dilution of 1:100. Blue: DAPI for nuclear staining.
3 / 4
Immunofluorescence analysis of NIH/3T3 cells using GPER1 antibody (13-509) at dilution of 1:100. Blue: DAPI for nuclear staining.
4 / 4
Immunofluorescence analysis of U2OS cells using GPER1 antibody (13-509) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using GPER1 antibody (13-509) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunofluorescence analysis of C6 cells using GPER1 antibody (13-509) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using GPER1 antibody (13-509) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using GPER1 antibody (13-509) at dilution of 1:100. Blue: DAPI for nuclear staining.

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PSI-13-509-100uL100 uL£565.00
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Further Information

Additional Names:
G-protein coupled estrogen receptor 1, Chemoattractant receptor-like 2, Flow-induced endothelial G-protein coupled receptor 1, FEG-1, G protein-coupled estrogen receptor 1, G-protein coupled receptor 30, GPCR-Br, IL8-related receptor DRY12, Lymphocyte-derived G-protein coupled receptor, LYGPR, Membrane estrogen receptor, mER, GPER1, CEPR, CMKRL2, DRY12, GPER, GPR30
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene is a member of the G-protein coupled receptor 1 family and encodes a multi-pass membrane protein that localizes to the endoplasmic reticulum. The protein binds estrogen, resulting in intracellular calcium mobilization and synthesis of phosphatidylinositol 3,4,5-trisphosphate in the nucleus. This protein therefore plays a role in the rapid nongenomic signaling events widely observed following stimulation of cells and tissues with estrogen. Alternate transcriptional splice variants which encode the same protein have been characterized.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-70 of human GPER1 (NP_001496.1).
NCBI Gene ID #:
2852
NCBI Official Name:
G-protein coupled estrogen receptor 1
NCBI Official Symbol:
GPER1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 52kDa
Purification:
Affinity purification
Research Area:
Neuroscience, Signal Transduction
Swissprot #:
Q99527
User NOte:
Optimal dilutions for each application to be determined by the researcher.