ProSci

TrkB Antibody

Product Code:
 
PSI-14-225
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 4
Western blot analysis of extracts of various cell lines, using TrkB Antibody (14-225) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
2 / 4
Immunohistochemistry of paraffin-embedded human lung cancer using TrkB antibody (14-225) at dilution of 1:200 (40x lens).
3 / 4
Immunohistochemistry of paraffin-embedded human placenta using TrkB antibody (14-225) at dilution of 1:200 (40x lens).
4 / 4
Immunohistochemistry of paraffin-embedded human gastric cancer using TrkB antibody (14-225) at dilution of 1:200 (40x lens).
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Western blot analysis of extracts of various cell lines, using TrkB Antibody (14-225) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human lung cancer using TrkB antibody (14-225) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human placenta using TrkB antibody (14-225) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using TrkB antibody (14-225) at dilution of 1:200 (40x lens).

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PSI-14-225-100uL100 uL£565.00
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Further Information

Additional Names:
NTRK2, neurotrophic tyrosine kinase, receptor, type 2, GP145-TrkB, TRKB , BDNF/NT-3 growth factors receptor, tyrosine kinase receptor B, BDNF receptor
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
This gene encodes a member of the neurotrophic tyrosine receptor kinase (NTRK) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Signalling through this kinase leads to cell differentiation. Mutations in this gene have been associated with obesity and mood disorders. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 41-433 of human TrkB (NP_001007098.1).
NCBI Gene ID #:
4915
NCBI Official Name:
neurotrophic tyrosine kinase, receptor, type 2
NCBI Official Symbol:
NTRK2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 110kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle, Growth Factors, Neuroscience, Signal Transduction
Swissprot #:
Q16620
User NOte:
Optimal dilutions for each application to be determined by the researcher.