ProSci

GYPC Antibody

Product Code:
 
PSI-14-362
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 2
Western blot analysis of extracts of various cell lines, using GYPC antibody (14-362) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 2
Immunofluorescence analysis of MCF-7 cells using GYPC antibody (14-362). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using GYPC antibody (14-362) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunofluorescence analysis of MCF-7 cells using GYPC antibody (14-362). Blue: DAPI for nuclear staining.

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PSI-14-362-100uL100 uL£565.00
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Further Information

Additional Names:
CD236, CD236R, GE, GE:GPC:GPD:GYPD, GPC, GPD, GYPD, PAS-2, PAS-2', glycophorin-C, glycoconnectin, glycophorin-D, glycoprotein beta, sialoglycoprotein D
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200
Background:
Glycophorin C (GYPC) is an integral membrane glycoprotein. It is a minor species carried by human erythrocytes, but plays an important role in regulating the mechanical stability of red cells. A number of glycophorin C mutations have been described. The Gerbich and Yus phenotypes are due to deletion of exon 3 and 2, respectively. The Webb and Duch antigens, also known as glycophorin D, result from single point mutations of the glycophorin C gene. The glycophorin C protein has very little homology with glycophorins A and B. Alternate splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-128 of human GYPC (NP_002092.1).
NCBI Gene ID #:
2995
NCBI Official Name:
glycophorin C (Gerbich blood group)
NCBI Official Symbol:
GYPC
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 34kDa
Purification:
Affinity purification
Research Area:
Immunology
Swissprot #:
P04921
User NOte:
Optimal dilutions for each application to be determined by the researcher.