Antibody Clonality:
Polyclonal
Storage:
Store at -20°C. Avoid freeze / thaw cycles.
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![Western blot analysis of extracts of various cell lines, using GYPC antibody (14-362) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s. Western blot analysis of extracts of various cell lines, using GYPC antibody (14-362) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.](https://www.prosci-inc.com/static-images/14-362_1.jpg)
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![Immunofluorescence analysis of MCF-7 cells using GYPC antibody (14-362). Blue: DAPI for nuclear staining. Immunofluorescence analysis of MCF-7 cells using GYPC antibody (14-362). Blue: DAPI for nuclear staining.](https://www.prosci-inc.com/static-images/14-362_2.jpg)
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This product comes from:
United States.
Typical lead time:
14-21 working days.
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Further Information
CD236, CD236R, GE, GE:GPC:GPD:GYPD, GPC, GPD, GYPD, PAS-2, PAS-2', glycophorin-C, glycoconnectin, glycophorin-D, glycoprotein beta, sialoglycoprotein D
WB: 1:500 - 1:2000
IF: 1:50 - 1:200
Glycophorin C (GYPC) is an integral membrane glycoprotein. It is a minor species carried by human erythrocytes, but plays an important role in regulating the mechanical stability of red cells. A number of glycophorin C mutations have been described. The Gerbich and Yus phenotypes are due to deletion of exon 3 and 2, respectively. The Webb and Duch antigens, also known as glycophorin D, result from single point mutations of the glycophorin C gene. The glycophorin C protein has very little homology with glycophorins A and B. Alternate splicing results in multiple transcript variants.
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
batch dependent
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Recombinant fusion protein containing a sequence corresponding to amino acids 1-128 of human GYPC (NP_002092.1).
2995
glycophorin C (Gerbich blood group)
GYPC
Homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 34kDa
Affinity purification
Immunology
P04921
Optimal dilutions for each application to be determined by the researcher.