ProSci

RPN1 Antibody

Product Code:
 
PSI-14-462
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of various cell lines, using RPN1 antibody (14-462) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded rat kidney using RPN1 antibody (14-462) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human tonsil using RPN1 antibody (14-462) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human liver cancer using RPN1 antibody (14-462) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human colon carcinoma using RPN1 antibody (14-462) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse testis using RPN1 antibody (14-462) at dilution of 1:100 (20x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using RPN1 antibody (14-462) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse kidney using RPN1 antibody (14-462) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of various cell lines, using RPN1 antibody (14-462) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat kidney using RPN1 antibody (14-462) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human tonsil using RPN1 antibody (14-462) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using RPN1 antibody (14-462) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using RPN1 antibody (14-462) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using RPN1 antibody (14-462) at dilution of 1:100 (20x lens).
Immunohistochemistry of paraffin-embedded mouse brain using RPN1 antibody (14-462) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using RPN1 antibody (14-462) at dilution of 1:100 (40x lens).

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CodeSizePrice
PSI-14-462-100uL100 uL£565.00
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Further Information

Additional Names:
RPN1, DKFZp686B16177, OST1, RBPH1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
This gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein forms part of the regulatory subunit of the 26S proteasome and may mediate binding of ubiquitin-like domains to this proteasome.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 20-280 of human RPN1 (NP_002941.1).
NCBI Gene ID #:
6184
NCBI Official Name:
ribophorin I
NCBI Official Symbol:
RPN1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 68kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
P04843
User NOte:
Optimal dilutions for each application to be determined by the researcher.