ProSci

TIA1 Antibody

Product Code:
 
PSI-14-482
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of various cell lines, using TIA1 antibody (14-482) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15s.
2 / 4
Immunohistochemistry of paraffin-embedded human colon carcinoma using TIA1 antibody (14-482) at dilution of 1:100 (40x lens).
3 / 4
Immunohistochemistry of paraffin-embedded human stomach using TIA1 antibody (14-482) at dilution of 1:100 (40x lens).
4 / 4
Immunofluorescence analysis of U2OS cells using TIA1 antibody (14-482) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using TIA1 antibody (14-482) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15s.
Immunohistochemistry of paraffin-embedded human colon carcinoma using TIA1 antibody (14-482) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using TIA1 antibody (14-482) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using TIA1 antibody (14-482) at dilution of 1:100. Blue: DAPI for nuclear staining.

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PSI-14-482-100uL100 uL£565.00
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Further Information

Additional Names:
TIA1, WDM, TIA-1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
The product encoded by this gene is a member of a RNA-binding protein family and possesses nucleolytic activity against cytotoxic lymphocyte (CTL) target cells. It has been suggested that this protein may be involved in the induction of apoptosis as it preferentially recognizes poly(A) homopolymers and induces DNA fragmentation in CTL targets. The major granule-associated species is a 15-kDa protein that is thought to be derived from the carboxyl terminus of the 40-kDa product by proteolytic processing. Alternative splicing resulting in different isoforms of this gene product has been described in the literature.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human TIA1 (NP_071505.2).
NCBI Gene ID #:
7072
NCBI Official Name:
TIA1 cytotoxic granule-associated RNA binding protein
NCBI Official Symbol:
TIA1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 43-50kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cell Cycle, Immunology
Swissprot #:
P31483
User NOte:
Optimal dilutions for each application to be determined by the researcher.