ProSci

EMR1 Antibody

Product Code:
 
PSI-14-508
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 3
Western blot analysis of extracts of various cell lines, using EMR1 antibody (14-508) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 3
Immunohistochemistry of paraffin-embedded mouse lung using EMR1 antibody (14-508) at dilution of 1:200 (40x lens).
3 / 3
Immunohistochemistry of paraffin-embedded mouse spleen using EMR1 antibody (14-508) at dilution of 1:200 (40x lens).

Western blot analysis of extracts of various cell lines, using EMR1 antibody (14-508) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded mouse lung using EMR1 antibody (14-508) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using EMR1 antibody (14-508) at dilution of 1:200 (40x lens).

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PSI-14-508-100uL100 uL£565.00
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This product comes from: United States.
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Further Information

Additional Names:
EMR1, TM7LN3, adhesion G protein-coupled receptor E1, EGF-like module receptor 1, EGF-like module-containing mucin-like hormone receptor-like 1, EMR1 hormone receptor, egf-like module containing, mucin-like, hormone receptor-like 1, egf-like module containing, mucin-like, hormone receptor-like sequence 1
Application Note:
WB: 1:500 - 1:1000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes a protein that has a domain resembling seven transmembrane G protein-coupled hormone receptors (7TM receptors) at its C-terminus. The N-terminus of the encoded protein has six EGF-like modules, separated from the transmembrane segments by a serine/threonine-rich domain, a feature reminiscent of mucin-like, single-span, integral membrane glycoproteins with adhesive properties. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 21-290 of human EMR1 (NP_001243182.1).
NCBI Gene ID #:
2015
NCBI Official Name:
adhesion G protein-coupled receptor E1
NCBI Official Symbol:
ADGRE1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 120kDa
Purification:
Affinity purification
Research Area:
Immunology, Innate Immunity, Signal Transduction
Swissprot #:
Q14246
User NOte:
Optimal dilutions for each application to be determined by the researcher.