ProSci

RUVBL2 Antibody

Product Code:
 
PSI-14-511
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of various cell lines, using RUVBL2 antibody (14-511) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 4
Immunohistochemistry of paraffin-embedded rat lung using RUVBL2 antibody (14-511) at dilution of 1:100 (40x lens).
3 / 4
Immunohistochemistry of paraffin-embedded mouse brain using RUVBL2 antibody (14-511) at dilution of 1:100 (40x lens).
4 / 4
Immunofluorescence analysis of MCF-7 cells using RUVBL2 antibody (14-511). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using RUVBL2 antibody (14-511) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat lung using RUVBL2 antibody (14-511) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using RUVBL2 antibody (14-511) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF-7 cells using RUVBL2 antibody (14-511). Blue: DAPI for nuclear staining.

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PSI-14-511-100uL100 uL£565.00
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Further Information

Additional Names:
RUVBL2, RVB2, TIH2, ECP51, TIP48, CGI-46, INO80J, REPTIN, TIP49B
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes the second human homologue of the bacterial RuvB gene. Bacterial RuvB protein is a DNA helicase essential for homologous recombination and DNA double-strand break repair. Functional analysis showed that this gene product has both ATPase and DNA helicase activities. This gene is physically linked to the CGB/LHB gene cluster on chromosome 19q13.3, and is very close (55 nt) to the LHB gene, in the opposite orientation.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-463 of human RUVBL2 (NP_006657.1).
NCBI Gene ID #:
10856
NCBI Official Name:
RuvB-like AAA ATPase 2
NCBI Official Symbol:
RUVBL2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 51kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
Q9Y230
User NOte:
Optimal dilutions for each application to be determined by the researcher.