ProSci

LGR4 Antibody

Product Code:
 
PSI-14-566
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 2
Western blot analysis of extracts of various cell lines, using LGR4 antibody (14-566) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
2 / 2
Immunofluorescence analysis of human placenta using LGR4 Polyclonal Antibody (14-566) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using LGR4 antibody (14-566) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Immunofluorescence analysis of human placenta using LGR4 Polyclonal Antibody (14-566) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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PSI-14-566-100uL100 uL£565.00
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Further Information

Additional Names:
BNMD17, GPR48, leucine-rich repeat-containing G-protein coupled receptor 4, G protein-coupled receptor 48
Application Note:
WB: 1:1000 - 1:3000

IF: 1:50 - 1:200
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 320-540 of human LGR4 (NP_060960.2).
NCBI Gene ID #:
55366
NCBI Official Name:
leucine rich repeat containing G protein-coupled receptor 4
NCBI Official Symbol:
LGR4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 105kDa
Purification:
Affinity purification
Research Area:
Signal Transduction
Swissprot #:
Q9BXB1
User NOte:
Optimal dilutions for each application to be determined by the researcher.