ProSci

WDR81 Antibody

Product Code:
 
PSI-14-624
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of Mouse brain, using WDR81 antibody (14-624) at 1:2000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 4
Immunofluorescence analysis of HeLa cells using WDR81 antibody (14-624) at dilution of 1:100. Blue: DAPI for nuclear staining.
3 / 4
Immunofluorescence analysis of NIH/3T3 cells using WDR81 antibody (14-624) at dilution of 1:100. Blue: DAPI for nuclear staining.
4 / 4
Immunofluorescence analysis of PC12 cells using WDR81 antibody (14-624) at dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of Mouse brain, using WDR81 antibody (14-624) at 1:2000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunofluorescence analysis of HeLa cells using WDR81 antibody (14-624) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using WDR81 antibody (14-624) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC12 cells using WDR81 antibody (14-624) at dilution of 1:100. Blue: DAPI for nuclear staining.

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PSI-14-624-100uL100 uL£565.00
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Further Information

Additional Names:
CAMRQ2, HYC3, PPP1R166, SORF-2, WD repeat-containing protein 81, protein phosphatase 1, regulatory subunit 166
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200
Background:
This gene encodes a multi-domain transmembrane protein, which is predominantly expressed in the brain. Mutations in this gene are associated with autosomal recessive cerebellar ataxia, mental retardation, and dysequilibrium syndrome-2. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 360-600 of human WDR81 (NP_689561.2).
NCBI Gene ID #:
124997
NCBI Official Name:
WD repeat domain 81
NCBI Official Symbol:
WDR81
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 260kDa
Purification:
Affinity purification
Research Area:
Cell Cycle
Swissprot #:
Q562E7
User NOte:
Optimal dilutions for each application to be determined by the researcher.