ProSci

GPC4 Antibody

Product Code:
 
PSI-14-635
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 4
Western blot analysis of extracts of various cell lines, using GPC4 antibody (14-635) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
2 / 4
Immunohistochemistry of paraffin-embedded human liver using GPC4 antibody (14-635) at dilution of 1:100 (40x lens).
3 / 4
Immunofluorescence analysis of HeLa cells using GPC4 antibody (14-635) at dilution of 1:100. Blue: DAPI for nuclear staining.
4 / 4
Immunofluorescence analysis of NIH/3T3 cells using GPC4 antibody (14-635) at dilution of 1:100. Blue: DAPI for nuclear staining.
❮❯

Western blot analysis of extracts of various cell lines, using GPC4 antibody (14-635) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human liver using GPC4 antibody (14-635) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using GPC4 antibody (14-635) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using GPC4 antibody (14-635) at dilution of 1:100. Blue: DAPI for nuclear staining.

No additional charges, what you see is what you pay! *

CodeSizePrice
PSI-14-635-100uL100 uL£565.00
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: United States.
Typical lead time: 14-21 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • Show All

Further Information

Additional Names:
GPC4, DJ900E8.1 (glypican 4), Glypican 4, Glypican proteoglycan 4, Glypican-4, K-glypican
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The GPC4 gene is adjacent to the 3' end of GPC3 and may also play a role in Simpson-Golabi-Behmel syndrome.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 357-556 of human GPC4 (NP_001439.2).
NCBI Gene ID #:
2239
NCBI Official Name:
glypican 4
NCBI Official Symbol:
GPC4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 70kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle, Growth Factors, Neuroscience, Signal Transduction, Stem Cell
Swissprot #:
O75487
User NOte:
Optimal dilutions for each application to be determined by the researcher.