ProSci

GBA2 Antibody

Product Code:
 
PSI-14-652
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of various cell lines, using GBA2 antibody (14-652) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
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Western blot analysis of extracts of various cell lines, using GBA2 antibody (14-652) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.

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PSI-14-652-100uL100 uL£565.00
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This product comes from: United States.
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Further Information

Additional Names:
AD035, NLGase, SPG46, non-lysosomal glucosylceramidase, beta-glucocerebrosidase 2, bile acid beta-glucosidase GBA2, bile acid glucosyl transferase GBA2, cholesterol glucosyltransferase GBA2, cholesteryl-beta-glucosidase GBA2, glucosidase, beta (bile acid) 2, glucosylceramidase 2
Application Note:
WB: 1:500 - 1:2000
Background:
This gene encodes a microsomal beta-glucosidase that catalyzes the hydrolysis of bile acid 3-O-glucosides as endogenous compounds. Studies to determine subcellular localization of this protein in the liver indicated that the enzyme was mainly enriched in the microsomal fraction where it appeared to be confined to the endoplasmic reticulum. This putative transmembrane protein is thought to play a role in carbohydrate transport and metabolism.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 708-927 of human GBA2 (NP_065995.1).
NCBI Gene ID #:
57704
NCBI Official Name:
glucosylceramidase beta 2
NCBI Official Symbol:
GBA2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 105kDa
Purification:
Affinity purification
Research Area:
Cancer, Signal Transduction
Swissprot #:
Q9HCG7
User NOte:
Optimal dilutions for each application to be determined by the researcher.