ProSci

USP30 Antibody

Product Code:
 
PSI-14-670
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 2
Western blot analysis of extracts of various cell lines, using USP30 antibody (14-670) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
2 / 2
Immunofluorescence analysis of NIH-3T3 cells using USP30 Polyclonal Antibody (14-670) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using USP30 antibody (14-670) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunofluorescence analysis of NIH-3T3 cells using USP30 Polyclonal Antibody (14-670) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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PSI-14-670-100uL100 uL£565.00
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Further Information

Additional Names:
ubiquitin carboxyl-terminal hydrolase 3deubiquitinating enzyme 3ub-specific protease 3ubiquitin thioesterase 3ubiquitin thiolesterase 3ubiquitin-specific protease 3ubiquitin-specific-processing protease 30
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:100
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 57-390 of human USP30 (NP_116052.2).
NCBI Gene ID #:
84749
NCBI Official Name:
ubiquitin specific peptidase 30
NCBI Official Symbol:
USP30
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 71kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle, Signal Transduction
Swissprot #:
Q70CQ3
User NOte:
Optimal dilutions for each application to be determined by the researcher.