ProSci

CDK7 Antibody

Product Code:
 
PSI-14-712
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of various cell lines, using CDK7 antibody (14-712) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15s.
2 / 4
Immunohistochemistry of paraffin-embedded human liver using CDK7 antibody (14-712) at dilution of 1:150 (40x lens).
3 / 4
Immunohistochemistry of paraffin-embedded human breast using CDK7 antibody (14-712) at dilution of 1:150 (40x lens).
4 / 4
Immunofluorescence analysis of HeLa cells using CDK7 Polyclonal Antibody (14-712) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using CDK7 antibody (14-712) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15s.
Immunohistochemistry of paraffin-embedded human liver using CDK7 antibody (14-712) at dilution of 1:150 (40x lens).
Immunohistochemistry of paraffin-embedded human breast using CDK7 antibody (14-712) at dilution of 1:150 (40x lens).
Immunofluorescence analysis of HeLa cells using CDK7 Polyclonal Antibody (14-712) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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PSI-14-712-100uL100 uL£686.00
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Further Information

Additional Names:
CDK7, CAK1, HCAK, MO15, STK1, CDKN7, p39MO15
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This protein forms a trimeric complex with cyclin H and MAT1, which functions as a Cdk-activating kinase (CAK). It is an essential component of the transcription factor TFIIH, that is involved in transcription initiation and DNA repair. This protein is thought to serve as a direct link between the regulation of transcription and the cell cycle.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 211-346 of human CDK7 (NP_001790.1).
NCBI Gene ID #:
1022
NCBI Official Name:
cyclin-dependent kinase 7
NCBI Official Symbol:
CDK7
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 39kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Signal Transduction
Swissprot #:
P50613
User NOte:
Optimal dilutions for each application to be determined by the researcher.