ProSci

USP36 Antibody

Product Code:
 
PSI-14-799
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 3
Western blot analysis of extracts of various cell lines, using USP36 antibody (14-799) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15s.
2 / 3
Immunofluorescence analysis of HeLa cells using USP36 Polyclonal Antibody (14-799) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
3 / 3
Immunofluorescence analysis of NIH-3T3 cells using USP36 Polyclonal Antibody (14-799) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using USP36 antibody (14-799) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15s.
Immunofluorescence analysis of HeLa cells using USP36 Polyclonal Antibody (14-799) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using USP36 Polyclonal Antibody (14-799) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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PSI-14-799-100uL100 uL£565.00
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Further Information

Additional Names:
DUB1, ubiquitin carboxyl-terminal hydrolase 36, deubiquitinating enzyme 36, ubiquitin specific protease 36, ubiquitin thioesterase 36, ubiquitin-specific-processing protease 36
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:100
Background:
This gene encodes a member of the peptidase C19 or ubiquitin-specific protease family of cysteine proteases. Members of this family remove ubiquitin molecules from polyubiquitinated proteins. The encoded protein may deubiquitinate and stabilize the transcription factor c-Myc, also known as MYC, an important oncoprotein known to be upregulated in most human cancers. The encoded protease may also regulate the activation of autophagy. This gene exhibits elevated expression in some breast and lung cancers.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human USP36 (NP_079366.3).
NCBI Gene ID #:
57602
NCBI Official Name:
ubiquitin specific peptidase 36
NCBI Official Symbol:
USP36
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 123kDa
Purification:
Affinity purification
Research Area:
Cell Cycle
Swissprot #:
Q9P275
User NOte:
Optimal dilutions for each application to be determined by the researcher.