ProSci

CTCF Antibody

Product Code:
 
PSI-14-881
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of 293T cells, using CTCF antibody (14-881) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
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Immunohistochemistry of paraffin-embedded rat liver using CTCF antibody (14-881) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded rat brain using CTCF antibody (14-881) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded rat spleen using CTCF antibody (14-881) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using CTCF antibody (14-881) at dilution of 1:200 (40x lens).
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Immunofluorescence analysis of U2OS cells using CTCF antibody (14-881) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug CTCF antibody (14-881). Western blot was performed from the immunoprecipitate using CTCF antibody (14-881) at a dilition of 1:1000.
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Chromatin immunoprecipitation analysis of extracts of HCT116 cells, using CTCF antibody (14-881) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
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Western blot analysis of extracts of 293T cells, using CTCF antibody (14-881) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
Immunohistochemistry of paraffin-embedded rat liver using CTCF antibody (14-881) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using CTCF antibody (14-881) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat spleen using CTCF antibody (14-881) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using CTCF antibody (14-881) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of U2OS cells using CTCF antibody (14-881) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug CTCF antibody (14-881). Western blot was performed from the immunoprecipitate using CTCF antibody (14-881) at a dilition of 1:1000.
Chromatin immunoprecipitation analysis of extracts of HCT116 cells, using CTCF antibody (14-881) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

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PSI-14-881-100uL100 uL£565.00
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Further Information

Additional Names:
CTCF, MRD21
Application Note:
WB: 1:1000 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200

IP: 1:50 - 1:100

ChIP: 1:50 - 1:200
Background:
This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human CTCF (NP_006556.1).
NCBI Gene ID #:
10664
NCBI Official Name:
CCCTC-binding factor (zinc finger protein)
NCBI Official Symbol:
CTCF
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 140kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
P49711
User NOte:
Optimal dilutions for each application to be determined by the researcher.