ProSci

HSP90AA1 Antibody

Product Code:
 
PSI-15-028
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 2
Western blot analysis of extracts of various cell lines, using HSP90AA1 antibody (15-028) at 1:500 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
2 / 2
Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug HSP90AA1 antibody (15-028). Western blot was performed from the immunoprecipitate using HSP90AA1 antibody (15-028) at a dilition of 1:500.
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Western blot analysis of extracts of various cell lines, using HSP90AA1 antibody (15-028) at 1:500 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug HSP90AA1 antibody (15-028). Western blot was performed from the immunoprecipitate using HSP90AA1 antibody (15-028) at a dilition of 1:500.

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PSI-15-028-100uL100 uL£565.00
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Further Information

Additional Names:
HSP90, HSP90A, HSPC1, HSPCA, Heat shock protein HSP 90-alpha, Heat shock 86 kDa, HSP 86, EL52, HSPN, LAP2, HSP86, Hsp89, Hsp90, HSP89A, HSP90N, HSPCAL1, HSPCAL4
Application Note:
WB: 1:500 - 1:2000

IP: 1:20 - 1:50
Background:
The protein encoded by this gene is an inducible molecular chaperone that functions as a homodimer. The encoded protein aids in the proper folding of specific target proteins by use of an ATPase activity that is modulated by co-chaperones. Two transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 433-732 of human HSP90AA1 (NP_005339.3).
NCBI Gene ID #:
3320
NCBI Official Name:
heat shock protein 90kDa alpha (cytosolic), class A member 1
NCBI Official Symbol:
HSP90AA1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 101kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cancer, Cell Cycle, Immunology, Signal Transduction
Swissprot #:
P07900
User NOte:
Optimal dilutions for each application to be determined by the researcher.