Antibody Clonality:
Polyclonal
Storage:
Store at -20°C. Avoid freeze / thaw cycles.
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![Western blot analysis of extracts of various cell lines, using PSMC5 antibody (15-052) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST. Western blot analysis of extracts of various cell lines, using PSMC5 antibody (15-052) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.](https://www.prosci-inc.com/static-images/15-052_1.jpg)
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![Immunohistochemistry of paraffin-embedded human lung cancer using PSMC5 antibody (15-052) at dilution of 1:100 (40x lens). Immunohistochemistry of paraffin-embedded human lung cancer using PSMC5 antibody (15-052) at dilution of 1:100 (40x lens).](https://www.prosci-inc.com/static-images/15-052_2.jpg)
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This product comes from:
United States.
Typical lead time:
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Further Information
PSMC5, S8, p45, SUG1, SUG-1, TBP10, TRIP1, p45/SUG
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases which have a chaperone-like activity. In addition to participation in proteasome functions, this subunit may participate in transcriptional regulation since it has been shown to interact with the thyroid hormone receptor and retinoid X receptor-alpha. Two transcript variants encoding different isoforms have been found for this gene.
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
batch dependent
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Recombinant fusion protein containing a sequence corresponding to amino acids 127-406 of human PSMC5 (NP_002796.4).
5705
proteasome (prosome, macropain) 26S subunit, ATPase, 5
PSMC5
Homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 45kDa
Affinity purification
Cell Cycle
P62195
Optimal dilutions for each application to be determined by the researcher.