ProSci

F3 Antibody

Product Code:
 
PSI-15-199
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 3
Western blot analysis of extracts of various cell lines, using F3 antibody (15-199) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
2 / 3
Immunohistochemistry of paraffin-embedded rat liver using F3 Antibody (15-199) at dilution of 1:100 (40x lens).
3 / 3
Immunohistochemistry of paraffin-embedded human esophagus using F3 Antibody (15-199) at dilution of 1:100 (40x lens).
❮❯

Western blot analysis of extracts of various cell lines, using F3 antibody (15-199) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunohistochemistry of paraffin-embedded rat liver using F3 Antibody (15-199) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using F3 Antibody (15-199) at dilution of 1:100 (40x lens).

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PSI-15-199-100uL100 uL£565.00
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Further Information

Additional Names:
CD142, TF, TFA, tissue factor, coagulation factor III (thromboplastin, tissue factor)
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces. There are 3 distinct domains of this factor: extracellular, transmembrane, and cytoplasmic. This protein is the only one in the coagulation pathway for which a congenital deficiency has not been described. Alternate splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 120-251 of human F3 (NP_001984.1).
NCBI Gene ID #:
2152
NCBI Official Name:
coagulation factor III, tissue factor
NCBI Official Symbol:
F3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 45kDa
Purification:
Affinity purification
Research Area:
Immunology
Swissprot #:
P13726
User NOte:
Optimal dilutions for each application to be determined by the researcher.