ProSci

GCC2 Antibody

Product Code:
 
PSI-15-215
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 5
Western blot analysis of extracts of various cell lines, using GCC2 antibody (15-215) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 5
Immunohistochemistry of paraffin-embedded human colon using GCC2 antibody (15-215) at dilution of 1:150 (40x lens).
3 / 5
Immunohistochemistry of paraffin-embedded human stomach using GCC2 antibody (15-215) at dilution of 1:150 (40x lens).
4 / 5
Immunohistochemistry of paraffin-embedded human appendix using GCC2 antibody (15-215) at dilution of 1:150 (40x lens).
5 / 5
Immunohistochemistry of paraffin-embedded mouse spleen using GCC2 antibody (15-215) at dilution of 1:150 (40x lens).
❮❯

Western blot analysis of extracts of various cell lines, using GCC2 antibody (15-215) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human colon using GCC2 antibody (15-215) at dilution of 1:150 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using GCC2 antibody (15-215) at dilution of 1:150 (40x lens).
Immunohistochemistry of paraffin-embedded human appendix using GCC2 antibody (15-215) at dilution of 1:150 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using GCC2 antibody (15-215) at dilution of 1:150 (40x lens).

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PSI-15-215-100uL100 uL£565.00
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Further Information

Additional Names:
GCC2, GRIP and coiled-coil domain containing 2, GCC185, KIAA0336, CLL-associated antigen KW-11, CTCL tumor antigen se1-1, GRIP and coiled-coil domain-containing 2, GRIP coiled-coil protein GCC185, (Ran-binding protein 2-like 4), RANBP2L4
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
The protein encoded by this gene is a peripheral membrane protein localized to the trans-Golgi network. It is sensitive to brefeldin A. This encoded protein contains a GRIP domain which is thought to be used in targeting. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human GCC2 (NP_852118.1).
NCBI Gene ID #:
9648
NCBI Official Name:
GRIP and coiled-coil domain containing 2
NCBI Official Symbol:
GCC2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 196kDa
Purification:
Affinity purification
Research Area:
Signal Transduction
Swissprot #:
Q8IWJ2
User NOte:
Optimal dilutions for each application to be determined by the researcher.