ProSci

PRMT5 Antibody

Product Code:
 
PSI-15-924
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 5
Western blot analysis of extracts of various cell lines, using PRMT5 antibody (15-924) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.
2 / 5
Immunohistochemistry of paraffin-embedded rat brain using PRMT5 antibody (15-924) at dilution of 1:100 (40x lens).
3 / 5
Immunohistochemistry of paraffin-embedded mouse brain using PRMT5 antibody (15-924) at dilution of 1:100 (40x lens).
4 / 5
Immunohistochemistry of paraffin-embedded human colon using PRMT5 antibody (15-924) at dilution of 1:100 (40x lens).
5 / 5
Immunofluorescence analysis of U2OS cells using PRMT5 antibody (15-924). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using PRMT5 antibody (15-924) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.
Immunohistochemistry of paraffin-embedded rat brain using PRMT5 antibody (15-924) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using PRMT5 antibody (15-924) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using PRMT5 antibody (15-924) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using PRMT5 antibody (15-924). Blue: DAPI for nuclear staining.

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PSI-15-924-100uL100 uL£565.00
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Further Information

Additional Names:
PRMT5, JBP1, SKB1, IBP72, SKB1Hs, HRMT1L5
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:200
Background:
This gene encodes an enzyme that belongs to the methyltransferase family. The encoded protein catalyzes the transfer of methyl groups to the amino acid arginine, in target proteins that include histones, transcriptional elongation factors and the tumor suppressor p53. This gene plays a role in several cellular processes, including transcriptional regulation, and the assembly of small nuclear ribonucleoproteins. A pseudogene of this gene has been defined on chromosome 4. Alternative splicing results in multiple transcript variants encoding different isoforms.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 40-140 of human PRMT5 (NP_006100.2).
NCBI Gene ID #:
10419
NCBI Official Name:
protein arginine methyltransferase 5
NCBI Official Symbol:
PRMT5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 70kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
O14744
User NOte:
Optimal dilutions for each application to be determined by the researcher.