ProSci

CANX Antibody

Product Code:
 
PSI-16-150
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 3
Western blot analysis of extracts of various cell lines, using CANX antibody (16-150) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
2 / 3
Immunofluorescence analysis of HeLa cells using CANX antibody (16-150) at dilution of 1:100. Blue: DAPI for nuclear staining.
3 / 3
Immunofluorescence analysis of NIH/3T3 cells using CANX antibody (16-150) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using CANX antibody (16-150) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
Immunofluorescence analysis of HeLa cells using CANX antibody (16-150) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using CANX antibody (16-150) at dilution of 1:100. Blue: DAPI for nuclear staining.

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PSI-16-150-100uL100 uL£565.00
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Further Information

Additional Names:
major histocompatibility complex class I antigen-binding protein p88, P90, IP90, FLJ26570, CNX, calnexin, CANX
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:100
Background:
This gene encodes a member of the calnexin family of molecular chaperones. The encoded protein is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Alternatively spliced transcript variants encoding the same protein have been described.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 501-592 of human CANX (NP_001737.1).
NCBI Gene ID #:
821
NCBI Official Name:
calnexin
NCBI Official Symbol:
CANX
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 100kDa
Purification:
Affinity purification
Research Area:
Neuroscience
Swissprot #:
P27824
User NOte:
Optimal dilutions for each application to be determined by the researcher.