ProSci

HNRNPD Antibody

Product Code:
 
PSI-16-177
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of various cell lines, using HNRNPD antibody (16-177) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
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Immunohistochemistry of paraffin-embedded rat liver using HNRNPD antibody (16-177) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human lung cancer using HNRNPD antibody (16-177) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using HNRNPD antibody (16-177) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of C6 cells using HNRNPD antibody (16-177) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of U-2 OS cells using HNRNPD antibody (16-177) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of C6 cells using HNRNPD antibody (16-177) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of U-2 OS cells using HNRNPD antibody (16-177) at dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using HNRNPD antibody (16-177) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunohistochemistry of paraffin-embedded rat liver using HNRNPD antibody (16-177) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using HNRNPD antibody (16-177) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using HNRNPD antibody (16-177) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using HNRNPD antibody (16-177) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using HNRNPD antibody (16-177) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of C6 cells using HNRNPD antibody (16-177) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using HNRNPD antibody (16-177) at dilution of 1:100. Blue: DAPI for nuclear staining.

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PSI-16-177-100uL100 uL£565.00
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This product comes from: United States.
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Further Information

Additional Names:
HNRPD, P37, AUF1, AUF1A, HNRPD, hnRNPD0
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are nucleic acid binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. It localizes to both the nucleus and the cytoplasm. This protein is implicated in the regulation of mRNA stability. Alternative splicing of this gene results in four transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-306 of human HNRNPD (NP_002129.2).
NCBI Gene ID #:
3184
NCBI Official Name:
heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa)
NCBI Official Symbol:
HNRNPD
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 37-45kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
Q14103
User NOte:
Optimal dilutions for each application to be determined by the researcher.