ProSci

PDPK1 Antibody

Product Code:
 
PSI-16-696
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 3
Western blot analysis of extracts of various cell lines, using PDPK1 antibody (16-696) at 1:500 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
2 / 3
Immunohistochemistry of paraffin-embedded rat brain using PDPK1 antibody (16-696) at dilution of 1:200 (40x lens).
3 / 3
Immunofluorescence analysis of U2OS cells using PDPK1 antibody (16-696). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using PDPK1 antibody (16-696) at 1:500 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat brain using PDPK1 antibody (16-696) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of U2OS cells using PDPK1 antibody (16-696). Blue: DAPI for nuclear staining.

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PSI-16-696-100uL100 uL£686.00
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Further Information

Additional Names:
PDPK1, MGC20087, MGC35290, PDK1, PRO0461, PkB-like, PkB-like 1, PDPK2
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 150-429 of human PDPK1 (NP_112558.2).
NCBI Gene ID #:
5170
NCBI Official Name:
3-phosphoinositide dependent protein kinase-1
NCBI Official Symbol:
PDPK1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 68kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cell Cycle, Immunology, Neuroscience, Signal Transduction
Swissprot #:
O15530
User NOte:
Optimal dilutions for each application to be determined by the researcher.