ProSci

USP21 Antibody

Product Code:
 
PSI-16-702
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of various cell lines, using USP21 antibody (16-702) at 1:1000 dilution. <br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Western blot analysis of extracts of various cell lines, using USP21 antibody (16-702) at 1:1000 dilution. <br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.

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PSI-16-702-100uL100 uL£565.00
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Further Information

Additional Names:
USP21, ubiquitin specific peptidase 21, MGC3394, USP16, USP23, NEDD8-specific protease, deubiquitinating enzyme 21, ubiquitin carboxyl-terminal hydrolase 21, ubiquitin specific protease 21, ubiquitin thiolesterase 21, ubiquitin-specific processing proteas, PP1490
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:100
Background:
This gene encodes a member of the C19 peptidase family, also known as family 2 of ubiquitin carboxy-terminal hydrolases. The encoded protein cleaves ubiquitin from ubiquitinated proteins for recycling in intracellular protein degradation. The encoded protein is also able to release NEDD8, a ubiquitin-like protein, from NEDD8-conjugated proteins. This gene has been referred to as USP16 and USP23 but is now known as USP21. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 400-500 of human USP21 (NP_001014443.1).
NCBI Gene ID #:
27005
NCBI Official Name:
ubiquitin specific peptidase 21
NCBI Official Symbol:
USP21
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 63kDa
Purification:
Affinity purification
Research Area:
Cell Cycle
Swissprot #:
Q9UK80
User NOte:
Optimal dilutions for each application to be determined by the researcher.