ProSci

SURF4 Antibody

Product Code:
 
PSI-16-739
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 3
Western blot analysis of extracts of various cell lines, using SURF4 antibody (16-739) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
2 / 3
Immunohistochemistry of paraffin-embedded human liver cancer using SURF4 antibody (16-739) at dilution of 1:200 (40x lens).
3 / 3
Immunohistochemistry of paraffin-embedded rat pancreatic islet using SURF4 antibody (16-739) at dilution of 1:200 (40x lens).
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Western blot analysis of extracts of various cell lines, using SURF4 antibody (16-739) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunohistochemistry of paraffin-embedded human liver cancer using SURF4 antibody (16-739) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat pancreatic islet using SURF4 antibody (16-739) at dilution of 1:200 (40x lens).

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PSI-16-739-100uL100 uL£565.00
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Further Information

Additional Names:
SURF4, ERV29, FLJ22993, MGC102753
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene is located in the surfeit gene cluster, which is comprised of very tightly linked housekeeping genes that do not share sequence similarity. The encoded protein is a conserved integral membrane protein that interacts with endoplasmic reticulum-Golgi intermediate compartment proteins. Disruption of this gene results in reduced numbers of endoplasmic reticulum-Golgi intermediate compartment clusters and redistribution of coat protein I to the cytosol. Alternate splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 50-150 of human SURF4 (NP_001267719.1).
NCBI Gene ID #:
6836
NCBI Official Name:
surfeit 4
NCBI Official Symbol:
SURF4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 30kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
O15260
User NOte:
Optimal dilutions for each application to be determined by the researcher.