ProSci

GLI2 Antibody

Product Code:
 
PSI-16-777
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 4
Western blot analysis of extracts of various cell lines, using GLI2 antibody (16-777) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
2 / 4
Immunofluorescence analysis of L-929 cells using GLI2 antibody (16-777) at dilution of 1:100. Blue: DAPI for nuclear staining.
3 / 4
Immunofluorescence analysis of U-2 OS cells using GLI2 antibody (16-777) at dilution of 1:100. Blue: DAPI for nuclear staining.
4 / 4
Immunofluorescence analysis of U-2 OS cells using GLI2 antibody (16-777) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using GLI2 antibody (16-777) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunofluorescence analysis of L-929 cells using GLI2 antibody (16-777) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using GLI2 antibody (16-777) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using GLI2 antibody (16-777) at dilution of 1:100. Blue: DAPI for nuclear staining.

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PSI-16-777-100uL100 uL£565.00
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Further Information

Additional Names:
GLI2, HPE9, THP1, THP2
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:100
Background:
This gene encodes a protein which belongs to the C2H2-type zinc finger protein subclass of the Gli family. Members of this subclass are characterized as transcription factors which bind DNA through zinc finger motifs. These motifs contain conserved H-C links. Gli family zinc finger proteins are mediators of Sonic hedgehog (Shh) signaling and they are implicated as potent oncogenes in the embryonal carcinoma cell. The protein encoded by this gene localizes to the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. It is also thought to play a role during embryogenesis. The encoded protein is associated with several phenotypes- Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial polydactyly type IV, postaxial polydactyly types A1 and B.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant protein of mouse GLI2.
NCBI Gene ID #:
14633
NCBI Official Name:
GLI family zinc finger 2
NCBI Official Symbol:
GLI2
NCBI Organism:
Mus musculus
Physical State:
Liquid
Purification:
Affinity purification
Research Area:
Cell Cycle, Neuroscience, Signal Transduction, Stem Cell, Transcription
Swissprot #:
P10070
User NOte:
Optimal dilutions for each application to be determined by the researcher.