ProSci

POLR2E Antibody

Product Code:
 
PSI-16-984
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 3
Western blot analysis of extracts of various cell lines, using POLR2E antibody (16-984) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
2 / 3
Immunohistochemistry of paraffin-embedded human stomach cancer using POLR2E antibody (16-984) at dilution of 1:100 (40x lens).
3 / 3
Immunofluorescence analysis of A549 cells using POLR2E antibody (16-984).
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Western blot analysis of extracts of various cell lines, using POLR2E antibody (16-984) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human stomach cancer using POLR2E antibody (16-984) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using POLR2E antibody (16-984).

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PSI-16-984-100uL100 uL£565.00
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Further Information

Additional Names:
DNA-directed RNA polymerases I, II, and III subunit RPABC1, RNA polymerases I, II, and III subunit ABC1, DNA-directed RNA polymerase II 23 kDa polypeptide, DNA-directed RNA polymerase II subunit E, RPB5 homolog, XAP4, POLR2E
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:200
Background:
This gene encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11. Three transcript variants encoding two different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human POLR2E (NP_002686.2).
NCBI Gene ID #:
5434
NCBI Official Name:
DNA-directed RNA polymerases I, II, and III subunit RPABC1
NCBI Official Symbol:
POLR2E
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 26kDa
Purification:
Affinity purification
Research Area:
Neuroscience
Swissprot #:
P19388
User NOte:
Optimal dilutions for each application to be determined by the researcher.