ProSci

ALDH1A1 Antibody

Product Code:
 
PSI-18-207
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of various cell lines, using ALDH1A1 antibody (18-207) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 20s.
2 / 4
Immunohistochemistry of paraffin-embedded rat liver using ALDH1A1 antibody (18-207) at dilution of 1:100 (40x lens).
3 / 4
Immunohistochemistry of paraffin-embedded human colon carcinoma using ALDH1A1 antibody (18-207) at dilution of 1:100 (40x lens).
4 / 4
Immunohistochemistry of paraffin-embedded mouse brain using ALDH1A1 antibody (18-207) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using ALDH1A1 antibody (18-207) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 20s.
Immunohistochemistry of paraffin-embedded rat liver using ALDH1A1 antibody (18-207) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using ALDH1A1 antibody (18-207) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using ALDH1A1 antibody (18-207) at dilution of 1:100 (40x lens).

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PSI-18-207-100uL100 uL£565.00
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Further Information

Additional Names:
Retinal dehydrogenase 1, RALDH 1, RalDH1, ALDH-E1, ALHDII, Aldehyde dehydrogenase family 1 member A1, Aldehyde dehydrogenase, cytosolic, ALDH1A1, ALDC, ALDH1, PUMB1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
The protein encoded by this gene belongs to the aldehyde dehydrogenase family. Aldehyde dehydrogenase is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. There are two major aldehyde dehydrogenase isozymes in the liver, cytosolic and mitochondrial, which are encoded by distinct genes, and can be distinguished by their electrophoretic mobility, kinetic properties, and subcellular localization. This gene encodes the cytosolic isozyme. Studies in mice show that through its role in retinol metabolism, this gene may also be involved in the regulation of the metabolic responses to high-fat diet.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 48-147 of human ALDH1A1 (NP_000680.2).
NCBI Gene ID #:
216
NCBI Official Name:
aldehyde dehydrogenase 1 family, member A1
NCBI Official Symbol:
ALDH1A1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 50-55kDa
Purification:
Affinity purification
Research Area:
Cancer, Neuroscience, Stem Cell
Swissprot #:
P00352
User NOte:
Optimal dilutions for each application to be determined by the researcher.