ProSci

HDAC2 Antibody

Product Code:
 
PSI-18-488
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of various cell lines, using HDAC2 antibody (18-488) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
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Immunohistochemistry of paraffin-embedded rat kidney using HDAC2 antibody (18-488) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human esophageal cancer using HDAC2 antibody (18-488) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse heart using HDAC2 antibody (18-488) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of A549 cells using HDAC2 antibody (18-488). Blue: DAPI for nuclear staining.
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Immunoprecipitation analysis of 200ug extracts of K562 cells using 1ug HDAC2 antibody (18-488). Western blot was performed from the immunoprecipitate using HDAC2 antibody (18-488) at a dilition of 1:1000.
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Western blot analysis of extracts of various cell lines, using HDAC2 antibody (18-488) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded rat kidney using HDAC2 antibody (18-488) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophageal cancer using HDAC2 antibody (18-488) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using HDAC2 antibody (18-488) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using HDAC2 antibody (18-488). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of K562 cells using 1ug HDAC2 antibody (18-488). Western blot was performed from the immunoprecipitate using HDAC2 antibody (18-488) at a dilition of 1:1000.

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PSI-18-488-100uL100 uL£565.00
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Further Information

Additional Names:
HDAC2 Antibody: HD2, RPD3, YAF1, Histone deacetylase 2, HD2
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:100

IP: 1:50 - 1:200
Background:
This gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). This protein forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 390 to the C-terminus of human HDAC2 (NP_001518.3).
NCBI Gene ID #:
3066
NCBI Official Name:
histone deacetylase 2
NCBI Official Symbol:
HDAC2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 60kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Immunology, Neuroscience, Signal Transduction, Stem Cell
Swissprot #:
Q92769
User NOte:
Optimal dilutions for each application to be determined by the researcher.