ProSci

DiMethyl-Histone H3-K79 pAb

Product Code:
 
PSI-18-631
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 7
Western blot analysis of extracts of various cell lines, using DiMethyl-Histone H3-K79 antibody (18-631).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
2 / 7
Dot-blot analysis of all sorts of methylation peptides using DiMethyl-Histone H3-K79 antibody (18-631).
3 / 7
Immunohistochemistry of paraffin-embedded human mammary cancer using DiMethyl-Histone H3-K79 antibody (18-631) at dilution of 1:200 (40x lens).
4 / 7
Immunohistochemistry of paraffin-embedded rat testis using DiMethyl-Histone H3-K79 antibody (18-631) at dilution of 1:200 (40x lens).
5 / 7
Immunohistochemistry of paraffin-embedded mouse testis using DiMethyl-Histone H3-K79 antibody (18-631) at dilution of 1:200 (40x lens).
6 / 7
Immunofluorescence analysis of 293T cells using DiMethyl-Histone H3-K79 antibody (18-631). Blue: DAPI for nuclear staining.
7 / 7
Chromatin Immunoprecipitation analysis of γ-actin gene from 293 cell line, using DiMethyl-Histone H3-K79 antibody (18-631) and rabbit IgG. P1, P2 and P3 were probes located on γ-actin gene as the schematic diagram illustrated. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
❮❯

Western blot analysis of extracts of various cell lines, using DiMethyl-Histone H3-K79 antibody (18-631).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Dot-blot analysis of all sorts of methylation peptides using DiMethyl-Histone H3-K79 antibody (18-631).
Immunohistochemistry of paraffin-embedded human mammary cancer using DiMethyl-Histone H3-K79 antibody (18-631) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat testis using DiMethyl-Histone H3-K79 antibody (18-631) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using DiMethyl-Histone H3-K79 antibody (18-631) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of 293T cells using DiMethyl-Histone H3-K79 antibody (18-631). Blue: DAPI for nuclear staining.
Chromatin Immunoprecipitation analysis of γ-actin gene from 293 cell line, using DiMethyl-Histone H3-K79 antibody (18-631) and rabbit IgG. P1, P2 and P3 were probes located on γ-actin gene as the schematic diagram illustrated. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

No additional charges, what you see is what you pay! *

CodeSizePrice
PSI-18-631-100uL100 uL£686.00
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: United States.
Typical lead time: 14-21 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • Show All

Further Information

Additional Names:
H3F3A, H3t, H3.4, H3/g, H3FT
Application Note:
WB: 1:500 - 1:1000

IHC: 1:50 - 1:100

IF: 1:50 - 1:200

IP: 1:50 - 1:200

ChIP: 1:50 - 1:200

CHIP seq: 1:50 - 1:200
Background:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide of human DiMethyl-Histone H3-K79
Modifications:
Methylated
NCBI Gene ID #:
8290
NCBI Official Name:
histone cluster 3, H3
NCBI Official Symbol:
H3K79me2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 17kDa
Purification:
Affinity purification
Research Area:
Signal Transduction
Swissprot #:
Q16695
User NOte:
Optimal dilutions for each application to be determined by the researcher.