ProSci

HDAC7 Antibody

Product Code:
 
PSI-18-889
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of HeLa cells, using HDAC7 antibody (18-889).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
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Immunohistochemistry of paraffin-embedded rat testis using HDAC7 antibody (18-889) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse liver using HDAC7 antibody (18-889) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse spinal cord using HDAC7 antibody (18-889) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of HeLa cells, using HDAC7 antibody (18-889).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded rat testis using HDAC7 antibody (18-889) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using HDAC7 antibody (18-889) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spinal cord using HDAC7 antibody (18-889) at dilution of 1:100 (40x lens).

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PSI-18-889-100uL100 uL£565.00
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Further Information

Additional Names:
Histone deacetylase 7, HD7, Histone deacetylase 7A, HD7a, HDAC7, HDAC7A
Application Note:
WB: 1:500 - 1:1000

IHC: 1:50 - 1:200
Background:
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene has sequence homology to members of the histone deacetylase family. This gene is orthologous to mouse HDAC7 gene whose protein promotes repression mediated via the transcriptional corepressor SMRT. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide of human HDAC7
NCBI Gene ID #:
51564
NCBI Official Name:
Histone deacetylase 7
NCBI Official Symbol:
HDAC7
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 65kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Immunology, Signal Transduction, Stem Cell
Swissprot #:
Q8WUI4
User NOte:
Optimal dilutions for each application to be determined by the researcher.