ProSci

BEST1 Antibody

Product Code:
 
PSI-18-909
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 4
Western blot analysis of extracts of various cell lines, using BEST1 antibody (18-909) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 4
Immunohistochemistry of paraffin-embedded rat brain using BEST1 antibody (18-909) at dilution of 1:100 (40x lens).
3 / 4
Immunohistochemistry of paraffin-embedded human liver damage using BEST1 antibody (18-909) at dilution of 1:100 (40x lens).
4 / 4
Immunohistochemistry of paraffin-embedded human stomach using BEST1 antibody (18-909) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of various cell lines, using BEST1 antibody (18-909) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat brain using BEST1 antibody (18-909) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver damage using BEST1 antibody (18-909) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using BEST1 antibody (18-909) at dilution of 1:100 (40x lens).

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PSI-18-909-100uL100 uL£565.00
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Further Information

Additional Names:
ARB, BEST, BMD, Best1V1Delta2, RP5TU15B, VMD2, bestrophin-1, Best disease, vitelliform macular dystrophy protein 2
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
This gene encodes a member of the bestrophin gene family. This small gene family is characterized by proteins with a highly conserved N-terminus with four to six transmembrane domains. Bestrophins may form chloride ion channels or may regulate voltage-gated L-type calcium-ion channels. Bestrophins are generally believed to form calcium-activated chloride-ion channels in epithelial cells but they have also been shown to be highly permeable to bicarbonate ion transport in retinal tissue. Mutations in this gene are responsible for juvenile-onset vitelliform macular dystrophy (VMD2), also known as Best macular dystrophy, in addition to adult-onset vitelliform macular dystrophy (AVMD) and other retinopathies. Alternative splicing results in multiple variants encoding distinct isoforms.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 200-300 of human BEST1 (NP_004174.1).
NCBI Gene ID #:
7439
NCBI Official Name:
bestrophin 1
NCBI Official Symbol:
BEST1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 70kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Neuroscience
Swissprot #:
O76090
User NOte:
Optimal dilutions for each application to be determined by the researcher.