ProSci

Histone H1.0 Antibody

Product Code:
 
PSI-19-025
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 4
Western blot analysis of extracts of various cell lines, using Histone H1.0 antibody (19-025) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 10s.
2 / 4
Immunohistochemistry of paraffin-embedded mouse brain using Histone H1.0 antibody (19-025) at dilution of 1:100 (40x lens).
3 / 4
Immunohistochemistry of paraffin-embedded human stomach using Histone H1.0 antibody (19-025) at dilution of 1:100 (40x lens).
4 / 4
Immunohistochemistry of paraffin-embedded rat liver using Histone H1.0 antibody (19-025) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of various cell lines, using Histone H1.0 antibody (19-025) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 10s.
Immunohistochemistry of paraffin-embedded mouse brain using Histone H1.0 antibody (19-025) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using Histone H1.0 antibody (19-025) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat liver using Histone H1.0 antibody (19-025) at dilution of 1:100 (40x lens).

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PSI-19-025-100uL100 uL£686.00
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Further Information

Additional Names:
H1F0, H10, H1FV, MGC5241
Application Note:
WB: 1:500 - 1:1000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-independent histone that is a member of the histone H1 family.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-105 of human H1F0 (NP_005309.1).
NCBI Gene ID #:
3005
NCBI Official Name:
H1 histone family, member 0
NCBI Official Symbol:
H1F0
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 30kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
P07305
User NOte:
Optimal dilutions for each application to be determined by the researcher.