ProSci

MAP1B Antibody

Product Code:
 
PSI-19-029
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 4
Western blot analysis of extracts of various cell lines, using MAP1B antibody (19-029) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
2 / 4
Immunofluorescence analysis of C6 cells using MAP1B antibody (19-029) at dilution of 1:100. Blue: DAPI for nuclear staining.
3 / 4
Immunofluorescence analysis of NIH/3T3 cells using MAP1B antibody (19-029) at dilution of 1:100. Blue: DAPI for nuclear staining.
4 / 4
Immunofluorescence analysis of U2OS cells using MAP1B antibody (19-029) at dilution of 1:100. Blue: DAPI for nuclear staining.
❮❯

Western blot analysis of extracts of various cell lines, using MAP1B antibody (19-029) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Immunofluorescence analysis of C6 cells using MAP1B antibody (19-029) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using MAP1B antibody (19-029) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using MAP1B antibody (19-029) at dilution of 1:100. Blue: DAPI for nuclear staining.

No additional charges, what you see is what you pay! *

CodeSizePrice
PSI-19-029-100uL100 uL£565.00
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: United States.
Typical lead time: 14-21 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • Show All

Further Information

Additional Names:
MAP1B, FUTSCH, MAP-1B, MAP5
Application Note:
WB: 1:200 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The product of this gene is a precursor polypeptide that presumably undergoes proteolytic processing to generate the final MAP1B heavy chain and LC1 light chain. Gene knockout studies of the mouse microtubule-associated protein 1B gene suggested an important role in development and function of the nervous system.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1820-2100 of human MAP1B (NP_005900.2).
NCBI Gene ID #:
4131
NCBI Official Name:
microtubule-associated protein 1B
NCBI Official Symbol:
MAP1B
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 350kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Neuroscience, Signal Transduction
Swissprot #:
P46821
User NOte:
Optimal dilutions for each application to be determined by the researcher.